Related Experiment Video
Updated: Nov 25, 2025
05:22
Identification of the Genes Involved in Stomatal Development via Epidermal Phenotype Scoring
Published on: January 20, 2023
2.0K
RSD1 Is Essential for Stomatal Patterning and Files in Rice
Qi Yu1, Liang Chen1, Wenqi Zhou1
1Key Laboratory of Cell Activities and Stress Adaptations, Ministry of Education, School of Life Sciences, Lanzhou University, Lanzhou, China.
Frontiers in Plant Science
|December 17, 2020
Summary
Researchers identified rice stomata developmental defect 1 (rsd1) gene, crucial for regulating stomatal density and preventing clustered stomata. This discovery aids in developing drought-resistant crops by controlling plant water transpiration.
Area of Science:
- Plant Biology
- Genetics
- Crop Science
Background:
- Stomatal density regulates plant gas exchange and water transpiration.
- Understanding genetic control of stomatal development is key for crop improvement.
Purpose of the Study:
- To characterize the function of the rice gene 'stomatal developmental defect 1' (RSD1) in stomatal development.
- To investigate the relationship between RSD1 and OsSDD1 in regulating stomatal patterning.
Main Methods:
- Forward genetics screen for rice mutants with altered stomatal density.
- Map-based cloning to identify the mutated gene.
- CRISPR-Cas9 to create OsSDD1 deletion mutants.
- Dehydration stress assays.
Main Results:
- Identified 'rice stomata developmental defect 1' (rsd1-1) mutant with decreased stomatal density and clustered stomata.
- RSD1 encodes a protein with DUF630 and DUF632 domains, involved in regulating gene expression during stomatal development.
- RSD1 and OsSDD1 are essential for inhibiting ectopic asymmetric cell divisions and preventing stomatal clustering.
- rsd1 mutants exhibit enhanced dehydration avoidance due to reduced stomatal density.
Conclusions:
- RSD1 and OsSDD1 play critical roles in rice stomatal development by controlling cell division patterns.
- Manipulating stomatal density through genes like RSD1 offers a strategy for developing drought-resistant crops.
More Related Videos
Related Concept Videos
02:04Regulation of Transpiration by Stomata
30.1K
During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.
30.1K
01:25Cell Signaling in Plants
6.0K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
6.0K
02:41Responses to Drought and Flooding
11.5K
Water plays a significant role in the life cycle of plants. However, insufficient or excess of water can be detrimental and pose a serious threat to plants.
11.5K
01:27C4 Pathway and CAM
47.8K
Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
C4 Pathway
The C4 pathway is used by plants such as...
47.8K
02:19Morphogenesis
29.4K
Plant morphogenesis—the development of a plant’s form and structure—involves several overlapping developmental processes, including growth and cell differentiation. Precursor cells differentiate into specific cell types, which are organized into the tissues and organ systems that make up the functional plant.
29.4K