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Updated: Nov 25, 2025

Multiplexing Focused Ultrasound Stimulation with Fluorescence Microscopy
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Pulsed Interleaved MINFLUX.

Luciano A Masullo1,2, Florian Steiner3, Jonas Zähringer3

  • 1Centro de Investigaciones en Bionanociencias (CIBION), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2390, C1425FQD, Ciudad Autónoma de Buenos Aires, Argentina.

Nano Letters
|December 18, 2020
PubMed
Summary
This summary is machine-generated.

We developed p-MINFLUX, a simplified single-molecule localization method offering 1-2 nm precision. This technique provides fluorescence lifetime data, enabling advanced imaging and tracking applications.

Keywords:
DNA origamifluorescencesingle-molecule spectroscopysuper-resolution microscopy

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Area of Science:

  • Biophysics
  • Optical Microscopy
  • Nanotechnology

Background:

  • Single-molecule localization microscopy (SMLM) enables super-resolution imaging.
  • Existing methods like MINFLUX offer high precision but can have complex setups.
  • There is a need for more accessible and versatile SMLM techniques.

Purpose of the Study:

  • Introduce p-MINFLUX, an enhanced implementation of the MINFLUX technique.
  • Demonstrate its performance in single-molecule localization nanoscopy and tracking.
  • Highlight the added value of fluorescence lifetime information.

Main Methods:

  • Utilized interleaved laser pulses for excitation foci delivery at maximum repetition rate.
  • Employed static and dynamic DNA origami model systems for validation.
  • Integrated fluorescence lifetime measurement capabilities.

Main Results:

  • Achieved 1-2 nm localization precision with 2000-1000 photon counts.
  • Demonstrated successful single-molecule localization and tracking.
  • Showcased the ability to acquire fluorescence lifetime data for multiplexing and super-resolved lifetime imaging.

Conclusions:

  • p-MINFLUX offers a simplified yet powerful approach to SMLM.
  • The integration of fluorescence lifetime enhances imaging capabilities.
  • p-MINFLUX has the potential to advance single-molecule imaging and analysis.