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Updated: Nov 24, 2025
Peptide-based Identification of Functional Motifs and their Binding Partners
Published on: June 30, 2013
Expression of a novel dual-functional polypeptide and its pharmacological action research
Xiaodan Li1, Xingkai Yang2, Muhammad Umar2
1State Key Laboratory of Medical Chemical Biology, Key Laboratory for Bioactive Materials of the Ministry of Education, College of Life Science, Nankai University, 300071 Tianjin, China; School of Mental Health, Jining Medical University, Jining, Shandong 272067, China.
Aims:
To develop a dual-functional medicine for hypoglycemic and anti-thrombus.
Main Methods:
The long-acting glucagon like peptide-1 (5×GLP-1) and nattokinase (NK) were cloned by SOE PCR and gained the GLP-1 and NK fusion polypeptide after transformed into E. coli. Use of mice models for the hypoglycemic and anti-thrombus activity of the fusion polypeptide. Balb/C mice were given the carrageenan by intraperitoneal injection to induce tail thrombus models. Type 2 diabetes mellitus mice model was used to research the hypoglycemic function of the fusion polypeptide.
Key Findings:
Results showed that the fusion polypeptide could significantly prevent thrombus formation after oral administration. Continuous administration for 15 days, fasting blood glucose levels of the experimental group decreased to nearly normal levels.
Significance:
The present study investigated the expression, purification and functional activity of the rolGLP-1 and NK fusion polypeptide, which provided a foundation for further studying the detailed pharmaceutical mechanism and drug development.
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