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Related Concept Videos

Clinical Applications of Epidermal Stem Cells01:19

Clinical Applications of Epidermal Stem Cells

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Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...
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Related Experiment Video

Updated: Nov 24, 2025

Growth of Human and Sheep Corneal Endothelial Cell Layers on Biomaterial Membranes
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Growth of Human and Sheep Corneal Endothelial Cell Layers on Biomaterial Membranes

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New developments in corneal endothelial cell replacement.

Daniele Spinozzi1, Alina Miron1, Marieke Bruinsma1

  • 1Netherlands Institute for Innovative Ocular Surgery, Rotterdam, The Netherlands.

Acta Ophthalmologica
|December 28, 2020
PubMed
Summary
This summary is machine-generated.

Cell-based therapies offer promising alternatives to traditional corneal transplantation for endothelial disorders. These approaches utilize expanded human corneal endothelial cells (hCEC) for transplantation, addressing donor tissue limitations.

Keywords:
corneacorneal endotheliumtissue engineeringtransplantation

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Development of a Noninvasive, Laser-Assisted Experimental Model of Corneal Endothelial Cell Loss
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Development of a Noninvasive, Laser-Assisted Experimental Model of Corneal Endothelial Cell Loss

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Area of Science:

  • Ophthalmology
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Corneal transplantation, including Descemet membrane endothelial keratoplasty (DMEK) and Descemet stripping (automated) endothelial keratoplasty (DS(A)EK), is vital for treating corneal endothelial disorders.
  • The primary limitation of current endothelial keratoplasty techniques is the scarcity of healthy donor corneal tissue.
  • Alternative strategies are crucial to expand the availability of endothelial grafts for patients.

Purpose of the Study:

  • This review focuses on recent advancements in cell-based approaches for corneal endothelial regeneration.
  • It explores methods for isolating and expanding human corneal endothelial cells (hCEC) in vitro.
  • The review discusses novel therapeutic strategies, including cell sheet transplantation and cell injection therapies.

Main Methods:

  • Isolation and in vitro expansion of human corneal endothelial cells (hCEC) from donor corneas.
  • Development and application of natural and bioengineered carriers for endothelial cell sheet transplantation.
  • Investigation of cell injection techniques as a potential therapeutic delivery method.

Main Results:

  • Cell-based approaches show potential to overcome donor tissue limitations in corneal transplantation.
  • Various carriers are being explored to support and deliver expanded hCEC for transplantation.
  • Endothelial cell injection represents a novel and promising therapeutic avenue.

Conclusions:

  • Cell-based therapies, including cell sheet and cell injection methods, are emerging as viable alternatives to conventional corneal transplantation.
  • These innovative approaches hold significant promise for improving treatment accessibility and outcomes for patients with corneal endothelial diseases.
  • Continued research into hCEC expansion and delivery systems is essential for clinical translation.