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Related Experiment Video

Updated: Nov 23, 2025

Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay

Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay

Published on: September 14, 2014

A Protocol for Studying HIV-1 Envelope Glycoprotein Function.

Sneha Ratnapriya¹, Angela Chov¹, Alon Herschhorn^1,2,3,4

¹Division of Infectious Diseases and International Medicine, Department of Medicine, University of Minnesota, Minneapolis, MN 55455, USA.

|December 30, 2020

Summary

This summary is machine-generated.

This study details a protocol to measure HIV-1 envelope glycoprotein (Env) sensitivity to various factors, aiding in understanding viral entry and informing vaccine development.

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Last Updated: Nov 23, 2025

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Published on: September 14, 2014

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Area of Science:

Virology
Immunology
Structural Biology

Background:

HIV-1 envelope glycoproteins (Envs) are crucial for viral entry by binding to CD4 receptors and CCR5/CXCR4 coreceptors.
Envs are the primary targets for neutralizing antibodies and a key focus in HIV vaccine development strategies.

Purpose of the Study:

To provide a detailed, step-by-step protocol for assessing HIV-1 Env sensitivity.
To facilitate the study of viral infectivity and the functional parameters of HIV-1 Env.

Main Methods:

The protocol involves measuring Env sensitivity to ligands, cold temperatures, and small molecules.
It also encompasses methods for studying HIV-1 viral infectivity.
The protocol aims to dissect various parameters influencing HIV-1 Env function.

Main Results:

The study presents a comprehensive protocol for analyzing HIV-1 Env properties.
This protocol enables detailed investigation into factors affecting viral entry mechanisms.
The methodology allows for a deeper understanding of Env function in the context of HIV-1.

Conclusions:

The developed protocol offers a standardized approach for HIV-1 Env characterization.
This resource is valuable for researchers in HIV/AIDS, vaccine development, and virology.
Further application of this protocol can advance our understanding of HIV-1 pathogenesis and therapeutic targets.