Ultrasensitive Nucleic Acid Assay Based on Cyclometalated Iridium(III) Complex with High Electrochemiluminescence...

This work developed a sensitive electrochemiluminescence (ECL) biosensor based on a cyclometalated iridium(III) complex ((bt)₂Irbza), which was synthesized for the first time. Annihilation, reductive-oxidative, and oxidative-reductive ECL behaviors of (bt)₂Irbza were investigated, respectively. The oxidative-reductive ECL intensity was the strongest compared with the other two, which showed 16.7 times relative ECL efficiency compared with commercial [Ru(bpy)₃]²⁺ under the same experimental conditions. Therefore, an ECL biosensing system with (bt)₂Irbza as the anodic luminophore was established for miRNA detection based on a closed bipolar electrode (BPE). Combined with both steric hindrance and catalytic effects induced by hemin/G-quadruplex in the cathodic reservoir of BPE that changed the Faraday current of the cathode and thus mediated the ECL intensity of (bt)₂Irbza in the anode of BPE, the ECL sensor stated an ultrahigh sensitivity for microRNA (miRNA-122) analysis with a detection limit of 82 aM.