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Related Experiment Video

Updated: Nov 23, 2025

Fish Sperm Assessment Using Software and Cooling Devices

Fish Sperm Assessment Using Software and Cooling Devices

Published on: July 28, 2018

[Internal quality control products for computer-assisted sperm analysis: Research and application].

Ming Ma¹, Ning Zhang¹, Dong-Mei Hao¹

¹Center of Reproductive Medicine, General Hospital of Northern Theater Command, Shenyang, Liaoning 110016, China.

Zhonghua Nan Ke Xue = National Journal of Andrology

|December 31, 2020

Summary

A self-made semen quality control product, cryopreserved in liquid nitrogen, effectively monitors computer-assisted sperm analysis (CASA) parameters. This method offers improved accuracy and precision for sperm concentration and motility analysis.

Keywords:

semen quality control sperm concentration sperm motion parameters computer-assisted sperm analysis

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Last Updated: Nov 23, 2025

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Published on: July 28, 2018

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Published on: November 28, 2018

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Area of Science:

Andrology
Reproductive Medicine
Laboratory Quality Control

Background:

Computer-assisted sperm analysis (CASA) is crucial for evaluating male fertility.
Internal quality control (QC) is essential for ensuring the accuracy and reliability of CASA results.
Commercially available QC products may have limitations in cost or availability.

Purpose of the Study:

To develop and validate a self-made semen quality control (QC) product for internal QC of CASA.
To assess the feasibility and effectiveness of cryopreserved semen QC for sperm analysis.
To compare the performance of the self-made QC product with commercially available options.

Main Methods:

A self-made semen QC product was prepared and cryopreserved in liquid nitrogen.
CASA systems were calibrated using both commercial and self-made QC products.
Sperm concentration, motility, and various motion parameters (VCL, VSL, VAP, LIN, WOB, STR) were analyzed over 50 days.
Makler counting plates were used for independent verification of concentration and motility.

Main Results:

The self-made QC product demonstrated low coefficients of variation (CV) for sperm concentration (5.42%) and motility (7.9%).
Motion parameters analyzed by CASA also showed acceptable CVs, with some as low as 2.15% (WOB).
The self-made product exhibited a smaller CV for sperm concentration compared to commercial QC products.

Conclusions:

Self-made internal QC products using liquid nitrogen cryopreservation are a feasible and effective tool for CASA.
This method ensures the accuracy and precision of CASA-derived sperm concentration and motion parameters.
The developed QC product offers a cost-effective and reliable alternative for routine laboratory use.