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Updated: Nov 23, 2025
Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19
Published on: February 16, 2022
Insights
This review critically evaluates instrumental tools for diagnosing Coronavirus Disease 2019 (COVID-19). It highlights essential diagnostic methods like PCR and ELISA for detecting viral particles and antibodies.
Area of Science:
- Medical Diagnostics
- Virology
- Public Health
Background:
- The Coronavirus Disease 2019 (COVID-19) pandemic exposed vulnerabilities in global healthcare systems.
- Urgent development of diagnostic tools was necessitated by the rapid spread of the virus.
Purpose of the Study:
- To critically review and evaluate instrumental diagnostic tools for COVID-19.
- To assess methods developed in response to the pandemic.
Main Methods:
- A comprehensive survey of current literature and scientific reports.
- Identification and description of key analytical and diagnostic methods.
- Analysis of principles, limitations, and cited evidence for each method.
Main Results:
- Discussion of Polymerase Chain Reaction (PCR), Loop-mediated Isothermal Amplification (LAMP), Lateral Flow Immunochromatography Assay (LFIA), Enzyme-Linked Immunosorbent Assay (ELISA), biosensors, and ChemiLuminescence Immunoassay (CLIA).
- Evaluation of these methods for detecting viral particles, antigens, and host antibodies in various biological samples (blood, serum, plasma, swabs).
Conclusions:
- COVID-19 highlighted the urgent need for sensitive instrumental diagnostic methods.
- Effective diagnosis is crucial for distinguishing COVID-19 from other viral diseases.
Abstract:
AbstractAIMS: Aims: The Coronavirus Disease 2019 (COVID-19) caused not only global pandemic, but it also pointed at unprepared health care systems and countermeasures were introduced under the pressure of urgent circumstances. This review is focused on discussion and critical evaluation of instrumental tools for COVID-19 diagnosis that were developed in the last months.
Methods:
Survey of actual literature and scientific reports was made. The most substantial analytical and diagnostical methods were identified and described. Principles and limitations of the methods are described, and actual papers are cited in this review.
Results:
Analytical and diagnostical methods like Polymerase Chain Reaction (PCR), Loop-mediated isothermal Amplification (LAMP), Lateral Flow Immunochromatography Assay (LFIA), Enzyme-Linked Immunosorbent Assay (ELISA), biosensors and ChemiLuminescence ImmunoAssay (CLIA) are discussed for assay of viral particles, antigens and specific host antibodies in blood, serum, plasma, nasopharyngeal swab and other samples in order to diagnose COVID-19.
Conclusions:
The Coronavirus Disease 2019 (COVID-19) is an emerging disease that has spread over the world since the end of year 2019. The global epidemic pointed at the necessity to introduce sensitive methods for instrumental diagnosis of COVID-19 and distinguishing it from the other viral diseases. (Tab. 2, Ref. 96).
