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Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

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Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
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Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19
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COVID-19 molecular level laboratory diagnoses.

M Pohanka

    Bratislavske Lekarske Listy
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    Summary

    This review critically evaluates instrumental tools for diagnosing Coronavirus Disease 2019 (COVID-19). It highlights essential diagnostic methods like PCR and ELISA for detecting viral particles and antibodies.

    Keywords:
    COVID-19Coronavirus Disease 2019ELISAantibodybiosensordiagnosisenzyme-linked immunosorbent assayimmunoassaypolymerase chain reactionsevere acute respiratory syndrome coronavirus 2 SARS-CoV-2.

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    Area of Science:

    • Medical Diagnostics
    • Virology
    • Public Health

    Background:

    • The Coronavirus Disease 2019 (COVID-19) pandemic exposed vulnerabilities in global healthcare systems.
    • Urgent development of diagnostic tools was necessitated by the rapid spread of the virus.

    Purpose of the Study:

    • To critically review and evaluate instrumental diagnostic tools for COVID-19.
    • To assess methods developed in response to the pandemic.

    Main Methods:

    • A comprehensive survey of current literature and scientific reports.
    • Identification and description of key analytical and diagnostic methods.
    • Analysis of principles, limitations, and cited evidence for each method.

    Main Results:

    • Discussion of Polymerase Chain Reaction (PCR), Loop-mediated Isothermal Amplification (LAMP), Lateral Flow Immunochromatography Assay (LFIA), Enzyme-Linked Immunosorbent Assay (ELISA), biosensors, and ChemiLuminescence Immunoassay (CLIA).
    • Evaluation of these methods for detecting viral particles, antigens, and host antibodies in various biological samples (blood, serum, plasma, swabs).

    Conclusions:

    • COVID-19 highlighted the urgent need for sensitive instrumental diagnostic methods.
    • Effective diagnosis is crucial for distinguishing COVID-19 from other viral diseases.