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DNA Microarrays

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Updated: Nov 23, 2025

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging

Published on: December 8, 2023

DNA Nanoswitch Barcodes for Multiplexed Biomarker Profiling.

Arun Richard Chandrasekaran¹, Molly MacIsaac^2,3,4, Javier Vilcapoma¹

¹The RNA Institute, University at Albany, State University of New York, New York, New York 12222, United States.

|January 4, 2021

Summary

This summary is machine-generated.

Researchers developed programmable DNA nanoswitches for multiplexed biomarker detection. This innovation allows simultaneous detection of up to six different biomarkers, including DNA, RNA, antibodies, and proteins, in a single assay.

Keywords:

DNA barcodes DNA nanoswitches biosensing diagnostics genotyping multiplexed detection

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Area of Science:

Biotechnology
Molecular Biology
Nanotechnology

Background:

Molecular biomarkers are crucial for clinical diagnostics, prognostics, and disease research.
Current methods face challenges in simultaneously detecting multiple and diverse biomarkers.
A need exists for comprehensive assays that provide a more complete picture for clinical evaluation and research.

Purpose of the Study:

To develop a novel method for multiplexed detection of multiple molecular biomarkers.
To enable simultaneous detection of diverse biomolecule types (DNA, RNA, antibody, protein) in a single assay.
To demonstrate the clinical utility of the developed assay for cancer biomarker detection.

Main Methods:

Programmable DNA nanoswitches were engineered for biomarker detection.
A barcoding system was implemented, generating unique signatures for biomarker combinations.
The method demonstrated "mixed multiplexing" for simultaneous detection of different biomolecule types.
The assay was validated using a prostate cancer biomarker panel in serum.

Main Results:

The DNA nanoswitch system achieved multiplexed detection of up to six biomarkers simultaneously.
Each combination of biomarkers produced a unique barcode signature, offering 64 possibilities.
"Mixed multiplexing" successfully detected diverse biomolecules (DNA, RNA, antibody, protein) in one assay.
The assay demonstrated potential for clinical application by detecting prostate cancer biomarkers in serum.

Conclusions:

Programmable DNA nanoswitches offer a powerful platform for highly multiplexed biomarker detection.
The "mixed multiplexing" capability expands the utility of the assay for complex biological samples.
This technology holds significant promise for advancing diagnostics and research through comprehensive molecular profiling.