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Adenosine triphosphate, polymyxin B and B16 cell-derived immunization induce anticancer response
Carlos Barrera-Avalos1,2, Javier Mena1, Ximena López1
1Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, USACH, Alameda 3363, Santiago, Chile.
Abstract:
Aim: Whole dead tumor cells can be used as antigen source and the induction of protective immune response could be enhanced by damage-associated molecular patterns. Materials & methods: We generated whole dead tumor cells called B16-immunogenic cell bodies (ICBs) from B16 melanoma cells by nutrient starvation and evaluated the in vivo antitumor effect of B16-ICBs plus ATP and polymyxin B (PMB). Results: The subcutaneous immunization with B16-ICBs + PMB + ATP a 50% of tumor-free animals and induced a significant delay in tumor growth in a prophylactic approach. These results correlated with maturation of bone marrow-derived dendritic cells and activation of T CD8+ lymphocytes in vitro. Conclusion: Altogether, ICB + ATP + PMB is efficient in inducing the antitumor efficacy of the whole dead tumor cells vaccine.
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