Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Phase-tuned acousto-optic spatial filter.

Optics letters·2024

Same author

Design and testing of a multi-electrode apodized acousto-optic filter for arbitrary polarized light.

Applied optics·2024

Same author

Independent multicolored bottle-beam generation using acousto-optic spatial shaping of a femtosecond laser beam.

Optics letters·2023

Same author

Analysis of Acousto-Optic Figure of Merit in KGW and KYW Crystals.

Materials (Basel, Switzerland)·2022

Same author

Characterization and alignment of acousto-optic devices using digital tailored RF waveforms.

Applied optics·2022

Same author

Ring-shaped optical trap based on an acousto-optic tunable spatial filter.

Optics letters·2021

Search research articles

Related Experiment Video

Updated: Nov 22, 2025

Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures

Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures

Published on: December 1, 2023

AOTF-based hyperspectral imaging phase microscopy.

Konstantin B Yushkov¹, Justine Champagne², Jean-Claude Kastelik²

¹National University of Science and Technology "MISIS", 4 Leninsky prospekt, Moscow, 119049, Russia.

Biomedical Optics Express

|January 7, 2021

Summary

A new hyperspectral imaging system enhances phase imaging microscopy using a spatial light modulator (SLM) and acousto-optic tunable filter (AOTF). This affordable add-on improves contrast for phase-only objects in biomedical research.

More Related Videos

Hyperspectral Imaging as a Tool to Study Optical Anisotropy in Lanthanide-Based Molecular Single Crystals

Hyperspectral Imaging as a Tool to Study Optical Anisotropy in Lanthanide-Based Molecular Single Crystals

Published on: April 14, 2020

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Published on: August 22, 2019

Related Experiment Videos

Last Updated: Nov 22, 2025

Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures

Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures

Published on: December 1, 2023

Hyperspectral Imaging as a Tool to Study Optical Anisotropy in Lanthanide-Based Molecular Single Crystals

Hyperspectral Imaging as a Tool to Study Optical Anisotropy in Lanthanide-Based Molecular Single Crystals

Published on: April 14, 2020

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Published on: August 22, 2019

Area of Science:

Optics and Photonics
Biomedical Imaging
Microscopy

Background:

Phase imaging microscopy offers advantages for biomedical research and clinical applications due to its compatibility with standard bright-field microscopes.
Incoherent illumination is a key feature for convenience and affordability in existing phase imaging setups.

Purpose of the Study:

To design and report a novel hyperspectral imaging system for phase imaging microscopy.
To enhance contrast generation for phase-only objects using spectral and spatial filtering.

Main Methods:

Integration of a spatial light modulator (SLM) and an acousto-optic tunable filter (AOTF).
System designed as an add-on module for standard optical microscopes.
Utilized incoherent diascopic sample illumination.
SLM and AOTF positioned in Fourier-conjugate planes for matched filtering.

Main Results:

Demonstrated a new hyperspectral imaging system for phase contrast enhancement.
The system effectively generates contrast from phase-only objects through spectral and spatial filtering.
The design allows for add-on integration with existing microscopy infrastructure.

Conclusions:

The developed hyperspectral imaging system offers an advanced solution for phase imaging microscopy.
The combination of SLM and AOTF provides a powerful method for contrast generation.
This technology holds potential for improved biomedical research and clinical diagnostics.