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Related Experiment Video

Updated: Nov 22, 2025

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
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Cell-Based Biosensor to Visualize Nitric Oxide Release from Living Cells for Toxicity Assessment.

Moritoshi Sato1, Yoshio Umezawa2

  • 1Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro-ku, Tokyo, Japan. cmsato@mail.ecc.u-tokyo.ac.jp.

Methods in Molecular Biology (Clifton, N.J.)
|January 10, 2021
PubMed
Summary

Researchers developed a sensitive cell-based fluorescent biosensor for visualizing nitric oxide (NO) release from living cells. This tool enables optical measurements of NO, a key molecule in various physiological and toxicological processes.

Keywords:
Cell-based biosensorFluorescence resonance energy transfer (FRET )Fluorescent biosensorLive cell imagingNitric oxide (NO)

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Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Biophysics

Background:

  • Fluorescence imaging is crucial for observing biomolecular dynamics in vivo.
  • Nitric oxide (NO) is a vital signaling molecule implicated in numerous physiological and toxicological pathways.
  • Availability of specific biosensors is key to advancing in vivo molecular studies.

Purpose of the Study:

  • To develop and describe a novel, highly sensitive, cell-based fluorescent biosensor.
  • To enable visualization and optical measurement of nitric oxide (NO) release from living cells.
  • To provide a method for studying NO dynamics in biological systems.

Main Methods:

  • Development of a cell-based fluorescent biosensor.
  • Utilizing fluorescence imaging techniques for NO detection.
  • Performing optical measurements of NO release from cultured cells.

Main Results:

  • A highly sensitive cell-based biosensor for NO was successfully created.
  • The biosensor allows for effective visualization of NO released by living cells.
  • Optical measurement protocols for NO release were established.

Conclusions:

  • The developed fluorescent biosensor offers a powerful tool for studying NO.
  • This method facilitates the observation of NO dynamics in real-time within living systems.
  • The biosensor enhances our capability to investigate the roles of NO in health and disease.