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Targeted urine proteomics in lupus nephritis - a meta-analysis
Ting Zhang1, Valeria Duran1, Kamala Vanarsa1
1Department of Biomedical Engineering, University of Houston , Houston, Texas, USA.
Expert Review of Proteomics
|January 11, 2021
Summary
Novel targeted proteomic analysis of urine identified 23 proteins elevated in lupus nephritis (LN). These biomarkers, including chemokines and proteins involved in angiogenesis, show promise for diagnosing and monitoring this kidney disease.
Area of Science:
- Proteomics
- Biomarker Discovery
- Renal Disease Research
Background:
- Proteomic techniques are crucial for identifying disease biomarkers.
- Targeted proteomic platforms offer high-throughput detection of low-abundance proteins.
- Urine is an increasingly important biofluid for monitoring kidney diseases like lupus nephritis (LN).
Purpose of the Study:
- To identify novel urinary biomarkers for lupus nephritis (LN) using high-throughput targeted proteomics.
- To perform a meta-analysis of existing targeted proteomic studies on LN urine.
- To correlate urinary findings with kidney tissue data.
Main Methods:
- Screened PubMed for targeted proteomic studies of LN urine (≥1000 proteins).
- Combined data from primary studies and performed meta-analysis.
- Cross-referenced urine proteomic data with renal single-cell RNA sequencing data from LN kidneys.
Main Results:
- Two high-throughput platforms interrogated LN urine for ≥1000 proteins.
- Twenty-three urine proteins were significantly elevated in active LN across studies.
- Elevated proteins include chemokines, angiogenesis, and extracellular matrix turnover proteins; Cathepsin S, CXCL10, FasL, ferritin, MIF, and resistin were also elevated in LN kidneys.
Conclusions:
- Targeted urinary proteomics has revealed multiple novel biomarkers for LN.
- These findings highlight potential new diagnostic and monitoring tools for lupus nephritis.
- Further validation in prospective cohorts and mechanistic studies are needed to confirm clinical utility.
