Related Experiment Video
Updated: Nov 21, 2025
Author Spotlight: Insights into the Use of Apple-Derived Cellulose Scaffolds for Bone Tissue Engineering
Published on: February 23, 2024
Osteogenic differentiation ability of human mesenchymal stem cells on Chitosan/Poly (Caprolactone)/nano beta
Nadeem Siddiqui1, Sanjay Madala1, Sreenivasa Rao Parcha2
1Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Guntur, Andhra Pradesh-522502, India.
Abstract:
Our work depicts the development and characterization of Chitosan/Poly (caprolactone)/nano beta-Tricalcium phosphate (CS/PCL/β-TCP) porous composite scaffolds by freeze drying method. Addition of PCL to CS/β-TCP composite scaffolds had significantly increased the compressive strength besides decelerating the degradation rate. Human mesenchymal stem cells (hMSCs) were chosen to assess in-vitro biocompatibility of the prepared scaffolds in terms of cell viability, cell attachment and proliferation by MTT assay, SEM and DNA Quantification assays respectively. Further, increased osteogenic differentiation assay results (Alkaline Phosphatase assay and Total calcium content) revealed the role of β-TCP in composite scaffolds. Altogether, results suggest the potentiality of prepared porous freeze dried composite scaffolds in bone tissue engineering applications.
