Contribution of Bone Marrow-Derived Mesenchymal Stem Cells to Healing of Pulmonary Contusion-Created Rats
Sabri Demir1, Ahmet Erturk1, Yasemin Dere Gunal2
1Department of Pediatric Surgery, Ankara Bilkent City Hospital, Children Hospital, Ankara, Turkey; Department of Pediatric Surgery, School of Medicine, Kirikkale University, Kirikkale, Turkey.
Insights
Bone marrow-derived mesenchymal stem cells (BM-MSCs) show promise in treating pulmonary contusion (PC) injuries in rats. Treatment with BM-MSCs significantly reduced lung injury scores, suggesting a potential alternative therapy for PC.
Area of Science:
- Regenerative Medicine
- Pulmonary Medicine
- Trauma Surgery
Background:
- Pulmonary contusion (PC) is a common thoracic injury in children following trauma.
- Bone marrow-derived mesenchymal stem cells (BM-MSCs) possess therapeutic properties for wound healing and various diseases.
- The potential of BM-MSCs in treating PC remains largely unexplored.
Purpose of the Study:
- To investigate the efficacy of BM-MSCs in promoting the healing of pulmonary contusion (PC) in a rat model.
- To evaluate the distribution and impact of BM-MSCs on lung injury following PC.
Main Methods:
- Forty-five male Wistar albino rats were divided into four groups.
- Pulmonary contusion was induced in three groups; one group received BM-MSCs post-injury.
- BM-MSCs were labeled with green fluorescent protein for tracking; lung injury scores were assessed after one week.
Main Results:
- BM-MSCs were significantly more concentrated in the lungs of rats treated with BM-MSCs post-PC compared to controls.
- The group receiving BM-MSCs for PC treatment exhibited a notably lower lung injury score than the spontaneous healing group.
Conclusions:
- Increased BM-MSC presence at the injury site correlates with reduced lung injury.
- BM-MSCs demonstrate a therapeutic effect on pulmonary contusion healing, suggesting their potential as an alternative treatment.
- BM-MSC therapy may offer a novel approach for managing diffuse PC, especially in high-risk patients.
Background:
The most common thoracic injury in children, resulting in trauma, is pulmonary contusion (PC). Bone marrow-derived mesenchymal stem cells (BM-MSCs) are used in wound healing and many other diseases. This study aims to examine the effects of BM-MSCs on PC healing in rats.
Materials And Methods:
A total of 45 male Wistar albino rats were used. Four groups were formed. BM-MSCs were labeled with the green fluorescent protein. PC was observed in the control group. In group II, PC occured and left to spontaneous healing. In group III, PC formed and BM-MSCs were given. In group IV, BM-MSCs were given without PC formation. Subjects were sacrificed 1 week later. Whether there was any difference in terms of BM-MSC involvement and lung injury score was investigated. Statistical analysis was performed using the Statistical Package for Social Sciences (SPSS), version 17.0, software (SPSS Inc., Chicago, IL), and p value of <0.05 was considered statistically significant.
Results:
BM-MSCs were collected much more in the lungs in group III than in group IV. Group III had a lower lung injury score value than group II.
Conclusion:
The greater involvement of the BM-MSCs in the injury site, and further reductions in lung injury score suggest that BM-MSCs are contributing to the healing of the injury. The use of BM-MSCs in risky patients with diffuse PC may be an alternative treatment to conventional methods.
