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Updated: Nov 21, 2025

Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins
Published on: June 14, 2018
Immunotherapy with NK cells: recent developments in gene modification open up new avenues
Lisa Marie Reindl1,2, Nawid Albinger1,2, Tobias Bexte1,2
1Children's Hospital, Goethe-University Frankfurt, Frankfurt am Main, Germany.
Abstract:
Chimeric antigen receptor (CAR)-T cell therapies have achieved remarkable success. However, application-related toxicities, such as cytokine release syndrome or neurotoxicity, moved natural killer (NK) cells into focus as novel players in immunotherapy. CAR-NK cells provide an advantageous dual killing-capacity by CAR-dependent and -independent mechanisms and induce few side effects. While the majority of trials still use CAR-T cells, CAR-NK cell trials are on the rise with 19 ongoing studies worldwide. This review illuminates the current state of research and clinical application of CAR-NK cells, as well as future developmental potential.
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