Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Energy Diagrams, Transition States, and Intermediates02:13

Energy Diagrams, Transition States, and Intermediates

18.8K
Free-energy diagrams, or reaction coordinate diagrams, are graphs showing the energy changes that occur during a chemical reaction. The reaction coordinate represented on the horizontal axis shows how far the reaction has progressed structurally. Positions along the x-axis close to the reactants have structures resembling the reactants, while positions close to the products resemble the products.  Peaks on the energy diagram represent stable structures with measurable lifetimes, while...
18.8K
Protein Networks02:26

Protein Networks

4.3K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
4.3K
Protein-protein Interfaces02:04

Protein-protein Interfaces

14.2K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
14.2K
Radical Reactivity: Overview01:11

Radical Reactivity: Overview

2.4K
Radicals, the highly reactive species, gain stability by undergoing three different reactions. The first reaction involves a radical-radical coupling, in which a radical combines with another radical, forming a spin‐paired molecule. The second reaction is between a radical and a spin‐paired molecule, generating a new radical and a new spin‐paired molecule. The third reaction is radical decomposition in a unimolecular reaction, forming a new radical and a spin‐paired...
2.4K
Coupled Reactions01:17

Coupled Reactions

9.7K
Cellular processes such as building and breaking down complex molecules occur through stepwise chemical reactions. Some of these chemical reactions are spontaneous and release energy, whereas others require energy to proceed. Cells often couple the energy-releasing reaction with the energy-requiring one to carry out important cell functions. 
Energy in adenosine triphosphate or ATP molecules is easily accessible to do work. ATP powers the majority of energy-requiring cellular reactions....
9.7K
Radical Reactivity: Intramolecular vs Intermolecular01:33

Radical Reactivity: Intramolecular vs Intermolecular

2.0K
Radical reactions can occur either intermolecularly or intramolecularly. In an intermolecular radical reaction, a nucleophilic radical adds to an electrophilic alkene or vice versa. In such reactions, the radical and generally the alkene, which is also called the radical trap, are two different molecules. Additionally, for such intermolecular reactions to occur, the radical trap must be active, present in an excess concentration, and the radical starting material must have a weak...
2.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-Precision Intrinsic Interactome Elucidation of Chimeric Antigen Receptors via Photocatalytic Micromapping (μMap-CAR).

Journal of the American Chemical Society·2026
Same author

Host metabolism can produce many indoles and phenols independently of the microbiome.

Nature metabolism·2026
Same author

Piecewise Stereoselective Assembly of Multisubstituted Alkenes.

Journal of the American Chemical Society·2026
Same author

Oxyalkylation of Alkenes via Triple Radical Sorting.

Journal of the American Chemical Society·2026
Same author

Molecular mechanisms of the MLL4 complex in H3K4 methylation and p53-dependent transcription activation.

Molecular cell·2026
Same author

Chemical biology tools for studying histone post-translational modifications.

Cell chemical biology·2026
Same journal

Fluorescent merocyanines: from fundamental properties to applications as molecular probes, in bioimaging and as emissive dye aggregates.

Chemical Society reviews·2026
Same journal

Direct impure water electrolysis at industrial scale.

Chemical Society reviews·2026
Same journal

Catalytic valorization of polyolefins: from catalysts and processes to reactors.

Chemical Society reviews·2026
Same journal

Designing stable π-radicals.

Chemical Society reviews·2026
Same journal

Antibacterial drug discovery: challenges and preclinical promises from synthetic small molecules.

Chemical Society reviews·2026
Same journal

Selective carbon-carbon bond cleavage involving alkene moieties.

Chemical Society reviews·2026
See all related articles

Related Experiment Video

Updated: Nov 21, 2025

Mapping Dysfunctional Protein-Protein Interactions in Disease
09:39

Mapping Dysfunctional Protein-Protein Interactions in Disease

Published on: October 24, 2025

241

Reactive intermediates for interactome mapping.

Ciaran P Seath1, Aaron D Trowbridge, Tom W Muir

  • 1Merck Center for Catalysis, Princeton University, Princeton, NJ 08544, USA. dmacmill@princeton.edu.

Chemical Society Reviews
|January 18, 2021
PubMed
Summary
This summary is machine-generated.

Understanding transient protein-protein interactions (PPIs) is crucial for disease treatment. New methods using reactive intermediates capture these interactions in situ, revealing cellular microenvironments and accelerating biological discovery.

More Related Videos

Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling
11:19

Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling

Published on: November 17, 2019

16.7K
Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes
07:22

Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes

Published on: January 12, 2024

4.0K

Related Experiment Videos

Last Updated: Nov 21, 2025

Mapping Dysfunctional Protein-Protein Interactions in Disease
09:39

Mapping Dysfunctional Protein-Protein Interactions in Disease

Published on: October 24, 2025

241
Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling
11:19

Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling

Published on: November 17, 2019

16.7K
Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes
07:22

Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes

Published on: January 12, 2024

4.0K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cellular Biology

Background:

  • Cellular processes rely on biomolecular interactions, especially protein-protein interactions (PPIs), which are fundamental to cell signaling.
  • Identifying weak or transient PPIs within a cellular environment is challenging using traditional methods like immunoprecipitation.

Purpose of the Study:

  • To review novel strategies for elucidating challenging protein-protein interactions.
  • To discuss the generation and application of reactive intermediates for mapping cellular interactomes.

Main Methods:

  • Utilizing in situ generation of high-energy intermediates to cross-link with neighboring proteins.
  • Capturing a snapshot of the local biomolecular environment (interactome).

Main Results:

  • Demonstrated a method to overcome limitations in identifying weak or transient PPIs.
  • Provided insights into the dynamic interplay of biomolecules within their native cellular context.

Conclusions:

  • Reactive intermediates offer a powerful approach to discovering new biology and understanding cellular physiology.
  • This strategy has the potential to significantly accelerate the study of PPIs and their role in disease.