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Updated: Nov 20, 2025

Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli
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Genetically encoding ε-N-benzoyllysine in proteins

Yanli Ji1, Conghui Ren, Hui Miao

  • 1State Key Laboratory and Institute of Elemento-Organic Chemistry, College of Chemistry, Nankai University, Tianjin 300071, China. wxuan@nankai.edu.cn.

Chemical Communications (Cambridge, England)
|January 21, 2021
PubMed
Summary

We genetically encoded benzoyl-lysine (BzK) into proteins, enabling histone modification and sirtuin debenzoylase activity analysis. This new method advances the study of post-translational modifications.

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