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Detection of Eosinophil Extracellular DNA Traps
Marina Valente Barroso1,2, Josiane Sabbadini Neves3
1Institute of Microbiology Paulo de Góes, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Methods in Molecular Biology (Clifton, N.J.)
|January 24, 2021
Summary
Researchers present simple methods to analyze extracellular DNA traps released by eosinophils and other leukocytes. These techniques aid in understanding immune responses and disease physiopathology.
Area of Science:
- Immunology
- Cell Biology
Background:
- Extracellular DNA traps (eDNA-ETs) are crucial in cell-mediated immune responses to inflammation.
- Understanding eDNA-ET release by leukocytes, like eosinophils, is vital for disease physiopathology.
- Existing methods for analyzing eDNA-ETs can be complex.
Purpose of the Study:
- To detail two accessible protocols for analyzing extracellular DNA traps.
- To enable both semiquantitative and qualitative assessment of eDNA-ETs released by human eosinophils.
- To provide tools applicable to various leukocyte types and other cells.
Main Methods:
- Fluorimetry-based protocol for semiquantitative analysis of eDNA-ETs.
- Fluorescence microscopy-based protocol for qualitative analysis of eDNA-ETs.
- Protocols specifically optimized for human eosinophils but adaptable.
Main Results:
- Successful demonstration of two distinct, user-friendly protocols for eDNA-ET analysis.
- Validation of fluorimetry for semiquantitative and fluorescence microscopy for qualitative assessment.
- Established methods applicable to eosinophils, neutrophils, and potentially other cell types.
Conclusions:
- The described protocols offer simple and effective means to study eDNA-ET release.
- These methods enhance the investigation of inflammatory processes and related diseases.
- The adaptability of these techniques broadens their utility in cell biology and immunology research.
