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Updated: Nov 20, 2025

Application of Membrane and Cell Wall Selective Fluorescent Dyes for Live-Cell Imaging of Filamentous Fungi
Published on: November 28, 2019
Dyeing fungi: amphotericin B based fluorescent probes for multiplexed imaging
Assel Baibek1, Muhammed Üçüncü2, Bryn Short3
1EaStChem School of Chemistry, The University of Edinburgh, Edinburgh, UK. mark.bradley@ed.ac.uk.
Abstract:
The clinically used antifungal polyene amphotericin B was conjugated, via the mycosamine and the aglycon moieties, to fluorophores. The Cy5 conjugated probe showed selective labelling of fungi in the presence of bacteria, allowing multiplexed imaging and identification of microbial species in a co-culture of fungi and Gram-positive and Gram-negative bacteria.
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