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Updated: Nov 20, 2025

Extraction and Purification of Polyphenols from Freeze-dried Berry Powder for the Treatment of Vascular Smooth Muscle Cells In Vitro
Published on: July 5, 2017
Actinidia arguta Pulp: Phytochemical Composition, Radical Scavenging Activity, and in Vitro Cells Effects
Diana Pinto1, Stefania Sut2, Stefano Dall'Acqua2
1REQUIMTE/LAQV, Polytechnic of Porto - School of Engineering, Rua Dr. António Bernardino de Almeida, 4249-015, Porto, Portugal.
Abstract:
Hardy kiwifruit (Actinidia arguta) is a highly appreciated exotic fruit endowed with outstanding bioactive compounds. The present work proposes to characterize the pulp from A. arguta organic fruits, emphasizing its radicals scavenging capacity and effects on intestinal cells (Caco-2 and HT29-MTX). The physicochemical properties and phenolic profile were also screened. The total phenolic and flavonoid contents (TPC and TFC, respectively) of pulp were 12.21 mg GAE/g on dry weight (DW) and 5.92 mg CE/g DW, respectively. A high antioxidant activity was observed (FRAP: 151.41 μmol FSE/g DW; DPPH: 12.17 mg TE/g DW). Furthermore, the pulp did not induce a toxic effect on Caco-2 and HT29-MTX cells viability up to 1000 μg/mL. Regarding in vitro scavenging capacity, the pulp revealed the highest scavenging power against NO. (IC50 =3.45 μg/mL) and HOCl (IC50 =12.77 μg/mL). These results emphasize the richness of A. arguta fruit pulp to be used in different food products.
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