Related Experiment Video
Updated: Nov 19, 2025
08:56
Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
11.3K
Complete genome sequence of ornithogalum virus 3
I Mostert1, R Bester1, D Aldrich1
1Department of Genetics, Stellenbosch University, Stellenbosch, South Africa.
Archives of Virology
|January 27, 2021
Summary
The complete genome of Ornithogalum virus 3 (OV3), a potyvirus infecting ornamental plants, was sequenced. This provides crucial data for understanding and managing virus diseases in commercial Ornithogalum cultivation.
Area of Science:
- Plant Virology
- Genomics
- Ornamental Horticulture
Background:
- Ornithogalum thyrsoides is a commercially valuable ornamental plant.
- Virus infections negatively impact the aesthetic quality of plants and cut flowers.
- Four Ornithogalum-infecting viruses are known, but the full genome of Ornithogalum virus 3 (OV3) was previously unavailable.
Purpose of the Study:
- To determine the complete genome sequence of Ornithogalum virus 3 (OV3).
- To analyze the predicted polyprotein processing and phylogenetic relationships of OV3.
- To confirm the classification of OV3 within the genus Potyvirus.
Main Methods:
- High-throughput sequencing (HTS) was used to obtain the complete OV3 genome sequence.
- Sanger sequencing was employed for validation.
- Bioinformatic analyses, including multiple sequence alignments and phylogenetic analysis, were performed.
Main Results:
- The complete genome sequence of OV3 was successfully determined.
- The polyprotein was predicted to yield 10 mature peptides with conserved Potyvirus domains.
- Phylogenetic analysis confirmed OV3 as a distinct species within the genus Potyvirus.
Conclusions:
- This study reports the first complete genome sequence of Ornithogalum virus 3 (OV3).
- The findings provide essential genomic information for Ornithogalum virus research and disease management.
- Genomic data supports the classification of OV3 as a separate species in the Potyvirus genus.
Related Concept Videos
01:29Viruses with RNA Genomes
397
RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
397
01:26Size and Structure of Viral Genomes
406
Viral genomes exhibit remarkable diversity in size, structure, and composition, influencing their replication strategies and interactions with host cells. These genomes consist of either DNA or RNA and may be linear or circular. Additionally, they can be single-stranded or double-stranded, with each configuration affecting how the virus propagates within a host. RNA viruses, for instance, generally have smaller genomes than DNA viruses, a factor that contributes to their high mutation rates and...
406
01:29Viruses of Archaea
283
Archaeal viruses play a crucial role in the ecosystems of extremophilic archaea, particularly those belonging to the phyla Euryarchaeota and Crenarchaeota. By shaping host evolution and facilitating gene transfer, these viruses influence microbial communities and contribute to genetic diversity in extreme environments. The archaea they infect thrive in acidic hot springs and hydrothermal vents characterized by high temperatures and low pH. Archaeal viruses exhibit remarkable structural...
283
01:44Complementary DNA
30.5K
Overview
30.5K
00:57Viral Recombination
24.4K
Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
24.4K
03:18Non-LTR Retrotransposons
12.6K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
12.6K