Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Reporter Genes

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Structural motif search across the protein universe with Folddisco.

Nature biotechnology·2026

Same author

An Embedded Trace Redistribution Layer with Rounded-Bottom Cu Geometry and Ti Capping for Enhanced Electromigration Reliability.

Micromachines·2026

Same author

Distinct autophagy impairment mechanisms of huntingtin aggregates with different polyQ lengths.

Cell chemical biology·2026

Same author

Spatiotemporal dynamics of β-arrestin-mediated Src activation in 5-HT7 receptor signaling pathway.

The FEBS journal·2026

Same author

Neck-to-knee dixon MRI thigh volume as a superior mass biomarker for Sarcopenia: evidence from the UK biobank.

NPJ digital medicine·2026

Same author

Functional and structural insights into interactions between β-Arrestin 1 and Gαs or Gαi1.

Nature communications·2026

Search research articles

Related Experiment Video

Updated: Nov 19, 2025

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

Genetically Encoded Biosensors Based on Fluorescent Proteins.

Hyunbin Kim^1,2, Jeongmin Ju^1,2, Hae Nim Lee^1,3

¹Brain Science Institute, Korea Institute of Science and Technology (KIST), Seoul 02792, Korea.

Sensors (Basel, Switzerland)

|January 28, 2021

Summary

This summary is machine-generated.

Genetically encoded biosensors using fluorescent proteins (FPs) enable real-time cellular process monitoring. This review details various FP biosensor strategies, aiding the design of novel tools for molecular dynamics research.

Keywords:

BiFC FRET circular permutation ddFP fluorescent protein fluorescent timer genetically encoded biosensor split FP

More Related Videos

Real-time In Vivo Recording of Arabidopsis Calcium Signals During Insect Feeding Using a Fluorescent Biosensor

Real-time In Vivo Recording of Arabidopsis Calcium Signals During Insect Feeding Using a Fluorescent Biosensor

Published on: August 15, 2017

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

Published on: April 27, 2018

Related Experiment Videos

Last Updated: Nov 19, 2025

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

Real-time In Vivo Recording of Arabidopsis Calcium Signals During Insect Feeding Using a Fluorescent Biosensor

Real-time In Vivo Recording of Arabidopsis Calcium Signals During Insect Feeding Using a Fluorescent Biosensor

Published on: August 15, 2017

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

Published on: April 27, 2018

Area of Science:

Biotechnology
Molecular Biology
Cell Biology

Background:

Genetically encoded biosensors utilizing fluorescent proteins (FPs) are essential for real-time monitoring of molecular dynamics.
Understanding cellular processes requires precise observation of molecular activities in space and time.
Effective biosensor design depends on selecting appropriate sensing strategies tailored to specific molecular events.

Purpose of the Study:

To review diverse sensing strategies for genetically encoded fluorescent protein (FP)-based biosensors.
To elucidate the principles behind various FP biosensor designs, including translocation, FRET, split FP reconstitution, pH sensitivity, and maturation speed.
To provide examples and critical considerations for developing novel FP biosensors.

Main Methods:

Literature review of genetically encoded biosensors based on fluorescent proteins.
Categorization and explanation of different sensing strategies (e.g., translocation, FRET, split FP, pH sensitivity, maturation speed).
Discussion of design principles and critical factors for each strategy.

Main Results:

Identification and description of multiple sensing strategies for FP-based biosensors.
Explanation of the underlying mechanisms for each strategy.
Presentation of representative examples illustrating the application of these strategies.

Conclusions:

The choice of sensing strategy is critical for successful FP biosensor development.
This review provides a framework for understanding and designing advanced FP biosensors.
The discussed strategies facilitate the real-time monitoring of complex cellular dynamics.