Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Multicompartment Models: Overview01:14

Multicompartment Models: Overview

360
Multicompartment models are mathematical constructs that depict how drugs are distributed and eliminated within the body. They segment the body into several compartments, symbolizing various physiological or anatomical areas connected through drug transfer processes such as absorption, metabolism, distribution, and elimination.
These models offer a more comprehensive representation of drug behavior in the body than one-compartment models. They accommodate the complexity of drug distribution,...
360
SBAR II: Application of SBAR01:14

SBAR II: Application of SBAR

5.3K
SBAR is an effective communication tool used by healthcare professionals to communicate patient information accurately. SBAR stands for Situation, Background, Assessment, and Recommendation. For a better understanding, an example is given below.
SBAR Report from a Nurse to a Health Care Provider
S: "Hello, Dr. Smith. This is Jane, RN, from the Med Surg unit. I am calling to tell you about Ms. White in Room 210, who is experiencing increased pain and redness at her incision site. Her recent...
5.3K
SBAR I: Understanding the Concept01:29

SBAR I: Understanding the Concept

5.5K
Effective communication among healthcare professionals during hand-off reporting is essential to delivering safe and continuous patient care. Common professional interactions include reports to healthcare team members, hand-off, and transfer reports. Nurses routinely report information to other healthcare team members and also urgently contact healthcare providers to report changes in patient status.
Standardized methods of communication have been developed to ensure that information is...
5.5K
Two-Compartment Open Model: Overview01:05

Two-Compartment Open Model: Overview

359
Multicompartmental models are crucial tools in pharmacokinetics, providing a framework to understand how drugs move within the body. The two-compartment model is a crucial subtype, segmenting the body into central and peripheral compartments. The central compartment represents areas with high blood flow, such as plasma and highly perfused organs like the kidneys and liver, while the peripheral compartment signifies tissues with lower blood flow, like adipose tissue and muscle tissue.
The...
359
Multimachine Stability01:25

Multimachine Stability

295
Multimachine stability analysis is crucial for understanding the dynamics and stability of power systems with multiple synchronous machines. The objective is to solve the swing equations for a network of M machines connected to an N-bus power system.
In analyzing the system, the nodal equations represent the relationship between bus voltages, machine voltages, and machine currents. The nodal equation is given by:
295

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction to "Cryo-EM Structure of the FtsH Periplasmic Domain Reveals Functional Dynamics".

ACS chemical biology·2026
Same author

Purified zymogens reveal mechanisms of snake venom metalloproteinase auto-activation.

eLife·2026
Same author

Fragment-Derived Nicotinic Acid Analogues Inhibit hCA III and Downregulate <i>CA3</i> Expression in HepG2 Cells.

Biomolecules·2026
Same author

The Future of Obesity Care: Exploring Synergies Between Metabolic Bariatric Surgery, Interventional Endoscopy and Pharmacotherapy.

Obesity surgery·2026
Same author

Strain-Mediated Lattice Reconstruction Enhances Ferromagnetism in Cr<sub>2</sub>Ge<sub>2</sub>Te<sub>6</sub>/WTe<sub>2</sub> van der Waals Heterobilayers.

Nano letters·2026
Same author

Cryo-EM Structure of the FtsH Periplasmic Domain Reveals Functional Dynamics.

ACS chemical biology·2026
Same journal

Shaping the future of bioscience education: trends, challenges and community insights.

Emerging topics in life sciences·2026
Same journal

Perceived impact of field trips on students' sense of community, skills and knowledge in Biosciences and Chemistry undergraduate degrees.

Emerging topics in life sciences·2026
Same journal

Clinical assessment meets laboratory science: adapting OSCE methodology for authentic biosciences evaluation in the age of generative AI.

Emerging topics in life sciences·2026
Same journal

A holistic approach to addressing the degree awarding gap: a perspective.

Emerging topics in life sciences·2026
Same journal

Integrating Smart Worksheets into mandatory pre-laboratory exercises increased exercise completion rates and laboratory test grades.

Emerging topics in life sciences·2026
Same journal

Marking the markers: evaluating the potential of professional development through collaborative marking circles.

Emerging topics in life sciences·2026
See all related articles

Related Experiment Video

Updated: Nov 19, 2025

The MultiBac Protein Complex Production Platform at the EMBL
13:51

The MultiBac Protein Complex Production Platform at the EMBL

Published on: July 11, 2013

16.3K

The MultiBac system: a perspective.

Imre Berger1,2, Christine Tölzer1, Kapil Gupta1

  • 1Bristol Synthetic Biology Centre BrisSynBio, Biomedical Sciences, School of Biochemistry, University of Bristol, 1 Tankard's Close, Bristol BSH 1TD, U.K.

Emerging Topics in Life Sciences
|February 1, 2021
PubMed
Summary
This summary is machine-generated.

The MultiBac system uses baculovirus expression for high-quality protein production in insect cells. Recent advancements enable applications in mammalian cells, genomic engineering, and vaccine development.

Keywords:
DNA deliverybaculovirusgene therapygenome engineeringmultiprotein complexesrecombinant expression

More Related Videos

Updated Protocol for the Assembly and Use of the Minibioreactor Array (MBRA)
09:38

Updated Protocol for the Assembly and Use of the Minibioreactor Array (MBRA)

Published on: September 5, 2025

491
Using the Open-Source MALDI TOF-MS IDBac Pipeline for Analysis of Microbial Protein and Specialized Metabolite Data
09:29

Using the Open-Source MALDI TOF-MS IDBac Pipeline for Analysis of Microbial Protein and Specialized Metabolite Data

Published on: May 15, 2019

20.0K

Related Experiment Videos

Last Updated: Nov 19, 2025

The MultiBac Protein Complex Production Platform at the EMBL
13:51

The MultiBac Protein Complex Production Platform at the EMBL

Published on: July 11, 2013

16.3K
Updated Protocol for the Assembly and Use of the Minibioreactor Array (MBRA)
09:38

Updated Protocol for the Assembly and Use of the Minibioreactor Array (MBRA)

Published on: September 5, 2025

491
Using the Open-Source MALDI TOF-MS IDBac Pipeline for Analysis of Microbial Protein and Specialized Metabolite Data
09:29

Using the Open-Source MALDI TOF-MS IDBac Pipeline for Analysis of Microbial Protein and Specialized Metabolite Data

Published on: May 15, 2019

20.0K

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Synthetic Biology

Background:

  • Baculovirus expression systems are established methods for producing proteins in insect cells.
  • The MultiBac system, developed for multiprotein complex production, has been widely adopted since 2004.
  • Continuous optimization has enhanced MultiBac's utility for diverse research and industrial applications.

Purpose of the Study:

  • To provide a comprehensive overview of the MultiBac system's evolution and capabilities.
  • To highlight recent developments and future directions in baculovirus-based expression and engineering.
  • To showcase the system's adaptability for producing complex proteins, targeting mammalian cells, and enabling genomic engineering.

Main Methods:

  • Utilizing baculovirus expression vectors for protein production in insect cells.
  • Developing tailored MultiBac genomes for specific applications like glycoprotein and VLP production.
  • Engineering baculovirions for mammalian cell targeting (MultiBacMam) and integrating CRISPR/Cas for genomic applications.
  • Applying synthetic biology principles for genome optimization and scalable manufacturing.

Main Results:

  • MultiBac facilitates high-quality, high-yield protein production, including complex multiprotein assemblies.
  • Modified MultiBac systems enable production of humanized glycoproteins, pharmaceutical targets, and virus-like particles (VLPs).
  • MultiBacMam extends applications to mammalian cells, tissues, and organisms, with CRISPR/Cas integration enabling large-scale genomic engineering.

Conclusions:

  • The MultiBac system has evolved into a versatile platform for protein production, complex assembly, and advanced biological applications.
  • Ongoing developments in synthetic biology and genome engineering promise further expansion of baculovirus system capabilities.
  • The system's adaptability supports diverse research needs, from structural biology to vaccine development and large-scale manufacturing.