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DNA barcoding: A different perspective to introducing undergraduate students to DNA sequence analysis.

Daniel J Erasmus1

  • 1Department of Chemistry and Biochemistry, University of Northern British Columbia, Prince George, Canada.

Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology
|February 2, 2021
PubMed
Summary

Undergraduate students learned DNA barcoding by extracting insect DNA, performing PCR, and sequencing. This exercise enabled species identification, offering a novel approach to DNA analysis education.

Keywords:
DNA barcodingDNA sequence databasesDNA sequencing

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Area of Science:

  • Biochemistry Education
  • Molecular Biology
  • Biodiversity Assessment

Background:

  • Biochemistry labs traditionally focus on human disease, biochemistry, and biotechnology.
  • Anthropogenic climate change drives a need for biodiversity quantification, particularly via molecular methods like DNA barcoding.

Purpose of the Study:

  • To introduce undergraduate students to DNA sequencing, analysis, and DNA barcoding.
  • To provide a practical laboratory exercise for exploring molecular approaches to biodiversity.

Main Methods:

  • DNA extraction from insect legs.
  • Polymerase Chain Reaction (PCR) amplification of the Cytochrome C oxidase I gene.
  • DNA gel electrophoresis, sequencing, and bioinformatic analysis using FinchTV, Genbank, and BOLD.

Main Results:

  • Successful amplification and sequencing of the target gene region.
  • Students analyzed DNA sequences to identify insect species.
  • Demonstrated the practical application of DNA barcoding for species identification.

Conclusions:

  • This 3-week lab exercise effectively teaches DNA sequencing and analysis in the context of DNA barcoding.
  • The exercise offers a valuable alternative approach for introducing students to DNA databases and molecular identification techniques.