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Swab pooling: A new method for large-scale RT-qPCR screening of SARS-CoV-2 avoiding sample dilution.
Ana Paula Christoff1, Giuliano Netto Flores Cruz1, Aline Fernanda Rodrigues Sereia1
1BiomeHub Biotechnologies, Florianópolis-SC, Brazil.
Plos One
|February 4, 2021
Summary
Swab pooling, a novel SARS-CoV-2 testing method, accurately detects the virus by pooling swabs at collection. This approach significantly increases lab capacity and reduces testing needs for large-scale screening.
Area of Science:
- Virology
- Molecular Diagnostics
- Public Health
Background:
- Standard SARS-CoV-2 pool testing can be affected by sample dilution.
- Existing methods involve pooling equal volumes from individually collected samples, which can introduce variability.
Purpose of the Study:
- To evaluate the analytical and diagnostic performance of a new swab pooling methodology for SARS-CoV-2 detection.
- To assess the impact of swab pooling on laboratory capacity and testing efficiency.
Main Methods:
- Swab pooling involves combining swabs directly at the time of sample collection.
- Paired analysis of pooled and individual samples was conducted.
- Performance was assessed using RT-qPCR assays on a large patient cohort.
Main Results:
- Swab pooling demonstrated no false-positives or false-negatives compared to individual testing.
- Mean Cq differences between individual and pool samples were minimal, indicating reliable detection.
- The method increased laboratory capacity by 4.4 times and reduced required tests by 77%.
Conclusions:
- Swab pooling is a reliable and efficient method for large-scale SARS-CoV-2 screening, particularly in low prevalence settings.
- This methodology effectively minimizes sample dilution effects inherent in traditional pool testing.
- The approach offers significant advantages in terms of laboratory throughput and cost reduction.
