Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Engineered chromogenic proteins with carbohydrate binding modules for advanced textile dyeing.

International journal of biological macromolecules·2026
Same author

Effect of Piperine Codelivery on the Oral Bioavailability of Cannabidiol: Insights from <i>In Vitro</i> Digestion and <i>In Vivo</i> Pharmacokinetics.

Journal of agricultural and food chemistry·2026
Same author

Amino-functionalized bimetallic MOF nanozyme <i>via</i> solvent-assisted ligand exchange for interference-free phenolic detection.

Analytical methods : advancing methods and applications·2026
Same author

Lignin-Based Nanoparticles Loaded with Essential Oils: Potential for Diabetic Foot Infection Management.

ACS materials Au·2026
Same author

Chitosan/Tripolyphosphate Nanoparticles Encapsulating Essential Oils as a New Class of Biopesticides: Structural Properties and Ecotoxicity Evaluation.

Environmental toxicology·2026
Same author

Toxicological profiling of nutraceutical-grade curcumin-loaded solid lipid nanoparticles using Caco-2 cells and zebrafish (Danio rerio) embryo models.

Toxicology in vitro : an international journal published in association with BIBRA·2026

Related Experiment Video

Updated: Nov 18, 2025

Easy Manipulation of Architectures in Protein-based Hydrogels for Cell Culture Applications
08:50

Easy Manipulation of Architectures in Protein-based Hydrogels for Cell Culture Applications

Published on: August 4, 2017

7.0K

Ohmic heating as a new tool for protein scaffold engineering.

Rui M Rodrigues1, Ricardo N Pereira1, António A Vicente1

  • 1CEB-Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal.

Materials Science & Engineering. C, Materials for Biological Applications
|February 6, 2021
PubMed
Summary

Ohmic heating (OH) offers novel protein functionalization for tissue engineering scaffolds. This method enhances cellular proliferation and metabolic activity, creating improved, tunable protein-based materials.

Keywords:
Cell proliferationElectric filedGelationScaffoldsTissue engineering

More Related Videos

Designing Silk-silk Protein Alloy Materials for Biomedical Applications
11:14

Designing Silk-silk Protein Alloy Materials for Biomedical Applications

Published on: August 13, 2014

18.7K
Preparation of Hydroxy-PAAm Hydrogels for Decoupling the Effects of Mechanotransduction Cues
11:31

Preparation of Hydroxy-PAAm Hydrogels for Decoupling the Effects of Mechanotransduction Cues

Published on: August 28, 2014

13.7K

Related Experiment Videos

Last Updated: Nov 18, 2025

Easy Manipulation of Architectures in Protein-based Hydrogels for Cell Culture Applications
08:50

Easy Manipulation of Architectures in Protein-based Hydrogels for Cell Culture Applications

Published on: August 4, 2017

7.0K
Designing Silk-silk Protein Alloy Materials for Biomedical Applications
11:14

Designing Silk-silk Protein Alloy Materials for Biomedical Applications

Published on: August 13, 2014

18.7K
Preparation of Hydroxy-PAAm Hydrogels for Decoupling the Effects of Mechanotransduction Cues
11:31

Preparation of Hydroxy-PAAm Hydrogels for Decoupling the Effects of Mechanotransduction Cues

Published on: August 28, 2014

13.7K

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Protein Chemistry

Background:

  • Ohmic heating (OH) is an emerging technology for controlling protein functionality.
  • Protein-based scaffolds are crucial for tissue engineering applications.
  • Current methods for scaffold production have limitations in controlling protein properties.

Purpose of the Study:

  • To investigate the use of Ohmic heating (OH) for producing protein-based scaffolds for tissue engineering.
  • To explore how OH processing parameters influence scaffold properties.
  • To evaluate the biological performance of OH-processed scaffolds.

Main Methods:

  • BSA/casein solutions were processed using Ohmic heating.
  • Cold-gelation was induced with Ca2+ to form scaffolds.
  • Scaffold properties (contact angle, swelling, porosity, modulus, degradation) were analyzed.
  • Electric field variations were applied during processing.
  • Human fibroblast cell adhesion and proliferation were assessed.

Main Results:

  • Ohmic heating induced protein denaturation and aggregation, enabling scaffold formation.
  • Scaffold stability was dependent on OH temperature and time.
  • Electric field variations altered scaffold functional properties.
  • OH-processed scaffolds supported human fibroblast adhesion and proliferation.
  • The production process yielded a non-cytotoxic material.

Conclusions:

  • Ohmic heating is a novel and effective method for producing tunable protein-based scaffolds.
  • OH processing parameters, including electric field, significantly impact scaffold characteristics.
  • Enhanced cellular proliferation and metabolic activity were observed with OH-assisted scaffold production.
  • This technique presents a promising alternative for advanced tissue engineering materials.