Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mandelonitrile beta-glucuronide: synthesis and characterization.

C Fenselau, S Pallante, R P Batzinger

    Science (New York, N.Y.)
    |November 11, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Oligopeptide fragmentation viewed as constant neutral loss.

    Journal of the American Society for Mass Spectrometry·2013
    Same author

    Endothermic ion molecule reactions.

    Journal of the American Society for Mass Spectrometry·2013
    Same author

    Fragmentation characteristic of glutathione conjugates activated by high-energy collisions.

    Journal of the American Society for Mass Spectrometry·2013
    Same author

    Proton affinities of polyglycines assessed by using the kinetic method.

    Journal of the American Society for Mass Spectrometry·2013
    Same author

    High-energy collision-induced dissociation of multiply charged polypeptides produced by electrospray.

    Journal of the American Society for Mass Spectrometry·2013
    Same author

    Interface for a four-sector mass spectrometer with a dual-purpose collision cell: High transmission at low to intermediate energies.

    Journal of the American Society for Mass Spectrometry·2013
    Same journal

    Erratum for the Research Article "Detecting supramolecular organic nanoparticles during heat wave".

    Science (New York, N.Y.)·2026
    Same journal

    Local signals, systemic decline.

    Science (New York, N.Y.)·2026
    Same journal

    The mechanics of liver regeneration.

    Science (New York, N.Y.)·2026
    Same journal

    Computing in a memory with physics.

    Science (New York, N.Y.)·2026
    Same journal

    Retraction.

    Science (New York, N.Y.)·2026
    Same journal

    Making time.

    Science (New York, N.Y.)·2026
    See all related articles

    Laetrile, or mandelonitrile beta-glucuronide, was synthesized and tested. Commercial Laetrile preparations contained amygdalin and neoamygdalin, and both these compounds and mandelonitrile glucuronide were found to be mutagenic.

    Area of Science:

    • Biochemistry
    • Pharmacology
    • Toxicology

    Background:

    • Laetrile, patented as mandelonitrile beta-glucuronide, is a compound investigated for therapeutic properties.
    • The precise composition and potential mutagenicity of Laetrile and its related compounds require thorough scientific evaluation.

    Purpose of the Study:

    • To synthesize and characterize mandelonitrile beta-glucuronide.
    • To analyze the composition of commercial Laetrile preparations.
    • To assess the mutagenic potential of synthesized mandelonitrile beta-glucuronide and related compounds.

    Main Methods:

    • Enzyme immobilization of rabbit liver uridine diphosphate-glucuronosyl transferase on beaded sepharose for synthesis.
    • Analysis using thin-layer chromatography, nuclear magnetic resonance, and gas chromatography-mass spectrometry.

    Related Experiment Videos

  • Mutagenicity testing using Salmonella typhimurium strains TA 98 and TA 100.

    • Main Results:

    • Mandelonitrile beta-glucuronide was successfully synthesized.
    • Commercial Laetrile preparations were found to contain amygdalin and neoamygdalin, lacking glucuronide.
    • Mandelonitrile, mandelonitrile glucuronide, and a mixture of amygdalin and neoamygdalin demonstrated mutagenic activity.

    Conclusions:

    • The synthesis and characterization of mandelonitrile beta-glucuronide were achieved.
    • Commercial Laetrile products may not contain the expected glucuronide compound.
    • Mandelonitrile, its glucuronide, and amygdalin/neoamygdalin mixtures exhibit mutagenic properties, raising safety concerns.