Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Amperometry: Overview01:10

Amperometry: Overview

1.1K
Amperometry is a technique commonly used to measure the concentration of specific analytes in a solution by monitoring the electric current generated during an electrochemical reaction. It involves applying a constant potential between a working electrode and a reference electrode to measure the resulting current, which is proportional to the concentration of the analyte. The Clark oxygen electrode operates based on this principle of amperometry. It consists of a cathode and an anode enclosed...
1.1K
Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

16.3K
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
16.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development of an Enzyme-Based Electrochemical Acetone Gas Sensor Printed on a Porous Polyimide Film.

ACS omega·2026
Same author

Convenient and sensitive detection of viable Escherichia coli employing a sequential reaction between antibody-enzyme complexes.

Biosensors & bioelectronics·2026
Same author

Rapid and convenient electrochemical hemoglobin detection in mouse feces employing a DNA aptamer to evaluate the severity of colitis in a mouse model.

Analytical methods : advancing methods and applications·2026
Same author

Electrochemical Impedance Spectroscopy Study of Water Uptake during the Removal of Polymer Coatings on Artworks.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Creation of an Engineered Oxygen-Insensitive L-Glutamate Oxidase for the Application of Electrochemical L-Glutamate Sensors.

International journal of molecular sciences·2026
Same author

A bioreference electrode; a reference electrode which shifts its redox potential symmetrically with the working electrode to develop reliable bioelectrochemical sensors.

Biosensors & bioelectronics·2026

Related Experiment Video

Updated: Nov 17, 2025

Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing
08:19

Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing

Published on: June 1, 2012

14.7K

Development of an Interdigitated Electrode-Based Disposable Enzyme Sensor Strip for Glycated Albumin Measurement.

Mika Hatada1, Noya Loew1, Junko Okuda-Shimazaki1

  • 1Joint Department of Biomedical Engineering, The University of North Carolina at Chapel Hill and North Carolina State University, Chapel Hill, NC 27599, USA.

Molecules (Basel, Switzerland)
|February 12, 2021
PubMed
Summary

This study developed a sensitive disposable enzyme sensor strip for glycated albumin (GA) using an interdigitated electrode (IDE). The IDE sensor offers superior sensitivity and reproducibility for diabetes mellitus glycemic control monitoring.

Keywords:
diabetes mellitusdisposable enzyme sensorfructosyl amino acid oxidaseglycated albumininterdigitated electrodepoint of care testing

More Related Videos

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation
13:42

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

12.1K
Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection
07:51

Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection

Published on: February 1, 2022

3.6K

Related Experiment Videos

Last Updated: Nov 17, 2025

Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing
08:19

Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing

Published on: June 1, 2012

14.7K
Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation
13:42

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

12.1K
Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection
07:51

Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection

Published on: February 1, 2022

3.6K

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Sensor Technology

Background:

  • Glycated albumin (GA) is a key marker for diabetes mellitus glycemic control.
  • Existing sensor technologies for GA measurement have limitations in sensitivity and reproducibility.
  • Interdigitated electrodes (IDEs) offer unique electrochemical properties for sensor development.

Purpose of the Study:

  • To develop a highly sensitive disposable enzyme sensor strip for glycated albumin (GA) measurement.
  • To utilize an interdigitated electrode (IDE) platform for enhanced sensor performance.
  • To evaluate the sensitivity, reproducibility, and detection limits of the developed IDE enzyme sensor.

Main Methods:

  • Fabrication of a disposable enzyme sensor strip utilizing an interdigitated electrode (IDE).
  • Characterization of IDE performance using a ferrocyanide/ferricyanide redox couple.
  • Measurement of fructosyl lysine, a protease digestion product of GA, using the IDE enzyme sensor.
  • Comparison of the IDE sensor's performance with previously reported screen-printed electrode sensors.

Main Results:

  • The IDE demonstrated superior electrochemical characteristics, reaching a steady state oxidation current rapidly.
  • The IDE enzyme sensor strip provided highly reproducible measurements of fructosyl lysine.
  • The developed sensor exhibited enhanced sensitivity (2.8 nA µM⁻¹) and a lower limit of detection (1.2 µM) compared to screen-printed electrode sensors.
  • Accurate measurement of GA samples at healthy and diabetic levels was achieved with high resolution.

Conclusions:

  • Interdigitated electrodes (IDEs) are suitable for developing highly sensitive disposable amperometric enzyme sensors.
  • The developed IDE enzyme sensor strip offers improved performance for GA measurement.
  • This technology has the potential for reliable and sensitive monitoring of glycemic control in diabetes mellitus.