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Updated: Nov 17, 2025

Lineage-reprogramming of Pericyte-derived Cells of the Adult Human Brain into Induced Neurons
Published on: May 12, 2014
Assessment of Pericyte Phenotype by Flow Cytometry
Nusrat S Khan1,2, Christopher C West3,4,5, Fiona Rossi3
1BHF Centre for Cardiovascular Science, Queen's Medical Research Institute, University of Edinburgh, Edinburgh, UK.
Abstract:
Pericytes are mural cells closely associated with endothelial cells in capillaries and microvessels. They are precursors of mesenchymal stem/stromal cells that have historically been retrospectively characterized in culture. We established a protocol, described in this chapter, to characterize and isolate pericytes from multiple human organs by flow cytometry and fluorescence-activated cell sorting. This prospective purification of pericytes brings us a step forward in the development of strategies for their use in the clinic.

