Related Experiment Video
Updated: Nov 17, 2025

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping
Published on: May 12, 2023
A stable uncompleted tetramolecular G-quadruplex formed by d(AGnA) under acidic condition
Xiaoxuan Xiang1, Ying Bao1, Likang Sun1
1State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, China.
Abstract:
In this work, the effects of terminal adenines on the formation and stability of tetramolecular G-quadruplexes (G4s) have been studied by electrospray ionization mass spectrometry (ESI-MS), UV, CD and NMR spectroscopy. Several evidences suggested that the sequences d(AGnA) (n = 4 or 5) form stable uncompleted tetramolecular G4 at acidic condition which is different from the canonical one in the neutral condition. In addition, hydrolysis of guanine has also been observed in acidic condition that may occur for unpaired strands rather than in complete G4. Thus, a new G4 topology containing incomplete G-quartet is proposed that is very stable and particularly presents in acidic ammonium ions solution. The information presented in this study provides the new insight on the polymorphism of G4s in acidic environment, which may help understand of the special role of adenines on the formation of G4s.
Related Concept Videos
EDTA: Chemistry and Properties
EDTA: Auxiliary Complexing Reagents
Formation of Complex Ions
1° Amines to Diazonium or Aryldiazonium Salts: Diazotization with NaNO2 Mechanism
Maxam-Gilbert Sequencing
Challenges of the Maxam-Gilbert Method
The...
Structure of Amines

