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TRPM8 channel inhibitor-encapsulated hydrogel as a tunable surface for bone tissue engineering
Tusar Kanta Acharya1,2, Satish Kumar3, Nikhil Tiwari1,2
1School of Biological Sciences, National Institute of Science Education and Research (NISER)-Bhubaneswar, Jatni, Khurda, 752050, Odisha, India.
Scientific Reports
|February 13, 2021
Summary
Researchers discovered that inhibiting the TRPM8 ion channel promotes bone mineralization. Using a novel hydrogel, they successfully guided stem cell differentiation into bone-forming cells, offering a new approach for bone tissue engineering.
Area of Science:
- Biomaterials Science
- Stem Cell Biology
- Regenerative Medicine
Background:
- Bone tissue engineering faces challenges in achieving efficient biomaterial-guided mineralization.
- Stem cells and osteoblasts express TRPM8, a calcium ion channel linked to bone formation.
Purpose of the Study:
- To investigate the role of TRPM8 in osteogenesis and mineralization.
- To develop a novel hydrogel system for controlled delivery of TRPM8 modulators for bone regeneration.
Main Methods:
- Utilized CMT:HEMA hydrogel, a carbohydrate polymer with a nanofiber structure.
- Coated the hydrogel with AMTB, a TRPM8 inhibitor.
- Assessed osteoblast and Bone Marrow-derived Mesenchymal Stem Cell Pool (BM-MSCP) behavior, including adhesion, growth, differentiation, and mineralization.
Main Results:
- TRPM8 expression was confirmed in osteoblasts and BM-MSCP.
- Inhibition of TRPM8 by AMTB led to increased osteogenic marker expression and enhanced mineralization.
- AMTB-coated CMT:HEMA hydrogel demonstrated dose-dependent induction of BM-MSCP differentiation into osteoblasts and subsequent mineralization.
Conclusions:
- AMTB-coated CMT:HEMA hydrogel serves as a tunable surface for bone tissue engineering.
- TRPM8 inhibition is a viable strategy to promote stem cell differentiation and bone mineralization.
- This approach holds potential for broad applications in biomedical sectors requiring bone regeneration.
