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Updated: Nov 17, 2025

Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy
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Double staining method for array tomography using scanning electron microscopy.

Eunjin Kim1, Jiyoung Lee1, Seulgi Noh2,3

  • 1National Instrumentation Center for Environmental Management, Seoul National University, Seoul, South Korea.

Applied Microscopy
|February 13, 2021
PubMed
Summary
This summary is machine-generated.

Scanning electron microscopy (SEM) enables nanometer-scale brain tissue analysis. A new post-staining method improves array tomography for cellular organelle imaging, offering advantages over conventional techniques.

Keywords:
Array tomographyDouble staining with uranyl acetate and leadScanning electron microscopy

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Area of Science:

  • Neuroscience
  • Microscopy
  • Cell Biology

Background:

  • Scanning electron microscopy (SEM) is crucial for high-resolution, large-area brain tissue analysis.
  • Studying cellular organelles in brain cells requires vast data at nanometer scales.
  • Array tomography is effective for large-area imaging, often using osmium-thiocarbohydrazide-osmium (OTO) or ferrocyanide-reduced osmium for membrane contrast.

Purpose of the Study:

  • To evaluate an alternative post-staining technique for conventional samples in array tomography.
  • To compare the advantages of this post-staining method against conventional en bloc staining techniques.

Main Methods:

  • Utilized array tomography for large-area imaging of brain tissue.
  • Applied a novel post-staining technique to conventional samples.
  • Compared the efficacy of post-staining versus en bloc staining for array tomography.

Main Results:

  • The post-staining technique was found to be a viable alternative for array tomography.
  • This method demonstrated advantages for imaging cellular structures at nanometer resolution.
  • Conventional samples, when post-stained, proved adequate for array tomography.

Conclusions:

  • Post-staining conventional samples offers an effective approach for array tomography.
  • This technique enhances the utility of SEM in neurobiological research.
  • The findings provide a valuable alternative for researchers studying brain tissue ultrastructure.