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Bacteria-based electro-optical platform for ampicillin detection in aquatic solutions.

Olga I Guliy1, Stella S Evstigneeva1, Victor D Bunin2

  • 1Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, 410049, Russia.

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Summary

A novel bacteria-based sensor using Pseudomonas putida TSh-18 and electro-optical analysis can detect ampicillin in aquatic solutions. This rapid method achieves a low detection limit, suitable for monitoring antibiotic residues.

Keywords:
AmpicillinAntibiotic determinationBiosensing systemsElectro-optical platform

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Area of Science:

  • Microbiology
  • Biosensing
  • Analytical Chemistry

Background:

  • Antibiotic residues in aquatic environments pose risks.
  • Accurate and rapid detection methods for antibiotics like ampicillin are needed.
  • Current detection methods may not meet regulatory standards for sensitivity.

Purpose of the Study:

  • To develop and validate a bacteria-based sensory system for ampicillin detection.
  • To assess the performance of Pseudomonas putida TSh-18 as a biosensor.
  • To establish a rapid, real-time method for monitoring ampicillin in aquatic solutions.

Main Methods:

  • Utilized Pseudomonas putida TSh-18 as a biological sensing element.
  • Employed an electro-optical sensor to detect changes in cell polarizability anisotropy.
  • Analyzed bacterial responses at 900 and 2100 kHz to assess cytoplasm and cell membrane states.
  • Performed control experiments using phase-contrast microscopy and microbiological tests.

Main Results:

  • Successfully detected ampicillin in the concentration range of 0.5–600 μg/mL.
  • Observed rapid changes in cell polarizability anisotropy correlating with ampicillin exposure.
  • Demonstrated ampicillin detection in the presence of kanamycin.
  • Achieved a lower limit of detection of 0.5 μg/mL, below EU maximum residue limits.

Conclusions:

  • Pseudomonas putida TSh-18 is a viable sensor for ampicillin detection.
  • Electro-optical analysis provides a rapid (10 min) and sensitive method for real-time ampicillin monitoring.
  • The developed biosensor meets and exceeds regulatory standards for antibiotic residue detection.