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Charting Metabolism Heterogeneity by Nanostructure Imaging Mass Spectrometry: From Biological Systems to Subcellular

Amelia Palermo1

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Nanostructure imaging mass spectrometry (NIMS) and NIMS with fluorinated gold nanoparticles (f-AuNPs) offer advanced methods for studying metabolism heterogeneity. These techniques provide ultrahigh sensitivity and broad metabolome coverage for biological systems.

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Nanostructure imaging mass spectrometrymetabolism heterogeneitymetabolomicssingle cell approachessubcellular metabolismsystems biology

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Area of Science:

  • Metabolomics
  • Biophysical Chemistry
  • Analytical Chemistry

Background:

  • Metabolism heterogeneity is crucial for understanding biological functions.
  • Existing mass spectrometry imaging (MSI) methods face challenges like high noise and limited metabolome coverage.
  • Untargeted metabolomics requires advanced spatial analysis techniques.

Purpose of the Study:

  • To explore the application of nanostructure imaging mass spectrometry (NIMS) for metabolism heterogeneity.
  • To highlight the advantages of NIMS with fluorinated gold nanoparticles (f-AuNPs) for comprehensive metabolite detection.
  • To provide a critical outlook on future applications of NIMS in biological systems.

Main Methods:

  • Utilizing nanostructure imaging mass spectrometry (NIMS).
  • Employing NIMS with fluorinated gold nanoparticles (f-AuNPs) for enhanced detection.
  • Applying MSI for spatial analysis of metabolites in biological samples.

Main Results:

  • NIMS and f-AuNPs offer heterogeneous metabolome coverage.
  • These methods provide ultrahigh sensitivity and high lateral resolution.
  • NIMS with f-AuNPs enables simultaneous detection of polar metabolites and lipids.

Conclusions:

  • NIMS and f-AuNPs are powerful tools for exploring metabolism heterogeneity.
  • These techniques facilitate systems-level interpretation of metabolic changes.
  • Future applications promise to reveal metabolic architecture in health and disease across various biological scales.