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Updated: Nov 17, 2025
In vivo Neuronal Calcium Imaging in C. elegans
Published on: April 10, 2013
In vivo imaging of a PVD neuron in Caenorhabditis elegans
Xinjian Wang1, Tingting Li1,2, Jiawen Hu1
1School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
Abstract:
The nematode Caenorhabditis elegans nociceptive PVD neurons have highly ordered dendritic branches, making this an ideal model to study the development and organization of dendrites. A ser-2-promoter-driven GFP reporter line wyIs592[ser-2prom-3p::myr-GFP] provides a comprehensive visualization of PVD anatomy. Here, we describe the detailed procedures for imaging a PVD neuron using wyIs592 at late L4 larval stage in vivo by confocal microscopy. This protocol can also be applied to imaging other cells in C. elegans. For complete details on the use and execution of this protocol, please refer to Feng et al. (2020).
