Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Reprogramming the diseased liver: antioxidant-engineered mRNA nanoparticles as microenvironment modulators in MAFLD.

Immunometabolism (Cobham, Surrey)·2026

Same author

SGK1-inhibition restores cardiac repolarization in LQT2 rabbits and LQT3 mice by reducing late sodium current.

Pharmacological research·2026

Same author

Recellularized Humanized Bioengineered Biatrial Model for Arrhythmia, Biological Pacemakers, and Optogenetic Studies.

Circulation research·2026

Same author

Effects of Bovine Milk-Derived Extracellular Vesicles on a 3D Intestinal Stromal Compartment.

Cells·2026

Same author

Towards safer iPSC-CM transplantation: steroid-sparing immunosuppression and arrhythmia prevention.

Cardiovascular research·2026

Same author

A Systemic Selective Modified mRNA Delivery Platform for Preventing Chemotherapy-Induced Cardiotoxicity.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Nov 16, 2025

Decellularization and Recellularization of Whole Livers

Decellularization and Recellularization of Whole Livers

Published on: February 4, 2011

Using Decellularization/Recellularization Processes to Prepare Liver and Cardiac Engineered Tissues.

Matteo Ghiringhelli¹, Yousef Abboud², Snizhanna V Chorna²

¹Sohnis Research laboratory for Cardiac Electrophysiology and Regenerative Medicine, the Rappaport Faculty of Medicine and Research Institute, Technion-Israel Institute of Technology, Haifa, Israel. matteo@campus.technion.ac.il.

Methods in Molecular Biology (Clifton, N.J.)

|February 19, 2021

Summary

This summary is machine-generated.

Decellularized tissues create scaffolds for regenerative medicine. These scaffolds preserve tissue structure and extracellular matrix, enabling enhanced engineered tissues for disease modeling and drug testing.

Keywords:

Cardiomyocytes Decellularization Engineer slice Hepatocytes Patch Recellularization Transplantation

More Related Videos

Procedure for Decellularization of Porcine Heart by Retrograde Coronary Perfusion

Procedure for Decellularization of Porcine Heart by Retrograde Coronary Perfusion

Published on: December 6, 2012

Procedure for Decellularization of Rat Livers in an Oscillating-pressure Perfusion Device

Procedure for Decellularization of Rat Livers in an Oscillating-pressure Perfusion Device

Published on: August 10, 2015

Related Experiment Videos

Last Updated: Nov 16, 2025

Decellularization and Recellularization of Whole Livers

Decellularization and Recellularization of Whole Livers

Published on: February 4, 2011

Procedure for Decellularization of Porcine Heart by Retrograde Coronary Perfusion

Procedure for Decellularization of Porcine Heart by Retrograde Coronary Perfusion

Published on: December 6, 2012

Procedure for Decellularization of Rat Livers in an Oscillating-pressure Perfusion Device

Procedure for Decellularization of Rat Livers in an Oscillating-pressure Perfusion Device

Published on: August 10, 2015

Area of Science:

Biomaterials Science
Regenerative Medicine
Tissue Engineering

Background:

Tissue engineering utilizes cell-seeded scaffolds for disease modeling, drug testing, and regenerative medicine.
Decellularized tissues/organs offer attractive scaffolds by preserving native extracellular matrix (ECM) and 3D structure.
This approach facilitates the creation of biomimetic environments for cell growth and tissue development.

Purpose of the Study:

To describe methods for creating viable scaffolds from decellularized heart and liver tissues.
To highlight the utility of these scaffolds in studying ECM biological factors.
To advance the generation of engineered tissues with improved regenerative capabilities.

Main Methods:

Decellularization of native heart and liver tissues to remove cellular components.
Preservation of the native three-dimensional (3D) structure and extracellular matrix (ECM) composition.
Recellularization of the prepared scaffolds to support seeded cell growth and function.

Main Results:

Viable scaffolds were successfully generated from decellularized heart and liver tissues.
The decellularization process maintained the integrity of the tissue's macroscopic and microscopic structures.
The resulting scaffolds provided appropriate biological signals and mechanical cues for seeded cells.

Conclusions:

Decellularized heart and liver scaffolds are valuable tools in tissue engineering.
These scaffolds enable the study and exploitation of ECM biological factors.
This methodology supports the development of engineered tissues with enhanced regenerative properties.