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Related Concept Videos

Intralumenal Vesicles and Multivesicular Bodies01:38

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Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
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A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain
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Outer Membrane Vesicle Induction and Isolation for Vaccine Development.

Melanie D Balhuizen1, Edwin J A Veldhuizen1, Henk P Haagsman1

  • 1Division of Infectious Diseases and Immunology, Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands.

Frontiers in Microbiology
|February 22, 2021
PubMed
Summary
This summary is machine-generated.

Outer membrane vesicles (OMVs) from Gram-negative bacteria show promise for vaccine development due to their surface antigens. This review examines OMV induction methods, their impact on vesicle properties, and challenges in creating effective OMV-based vaccines.

Keywords:
Bordetella pertussisNeisseria meningitidishost defense peptidesinductionisolationouter membrane vesiclesvaccine development

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Area of Science:

  • Microbiology
  • Immunology
  • Biotechnology

Background:

  • Gram-negative bacteria release outer membrane vesicles (OMVs) involved in various cellular functions.
  • OMVs are promising vaccine candidates as they display bacterial surface antigens.
  • Current challenges include difficulties in OMV isolation and low yields.

Purpose of the Study:

  • To review current knowledge on outer membrane vesicles (OMVs).
  • To discuss methods for inducing and isolating OMVs, analyzing their advantages and disadvantages.
  • To compare the effects of different induction and isolation techniques on OMVs in immunological studies.

Main Methods:

  • Literature review of OMV induction and isolation methods.
  • Comparison of OMV properties based on different production techniques.
  • Analysis of immunological studies utilizing OMVs.

Main Results:

  • Various methods exist to induce OMV production, but they can alter vesicle characteristics.
  • OMV yield and purity are significantly impacted by the chosen induction and isolation protocols.
  • The effectiveness of OMVs in vaccine development is linked to the methods used for their production.

Conclusions:

  • Standardizing OMV production methods is crucial for consistent vaccine development.
  • Further research is needed to overcome challenges in OMV isolation and yield enhancement.
  • Successful OMV-based vaccines require careful consideration of induction and purification strategies.