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An Intestine/Liver Microphysiological System for Drug Pharmacokinetic and Toxicological Assessment

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Reference Intervals for Intestinal Disaccharidase Activities Determined from a Non-Reference Population.

Sarah A Hackenmueller¹, David G Grenache^1,2

¹Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT.

The Journal of Applied Laboratory Medicine

|February 25, 2021

Summary

This summary is machine-generated.

This study validated historical disaccharidase cutoffs using clinical data, finding lactase deficiency most common in pediatric patients. Updated cutoffs for palatinase and lactase align with calculated reference intervals for improved diagnostic accuracy.

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Area of Science:

Gastroenterology
Clinical Chemistry
Pediatric Medicine

Background:

Historical disaccharidase activity cutoffs lack verification from reference populations.
This study addresses the need to validate existing diagnostic criteria for disaccharidase deficiencies.
Understanding patient demographics undergoing intestinal disaccharidase testing is crucial.

Purpose of the Study:

To validate historical disaccharidase activity cutoffs using clinical sample data.
To establish accurate reference intervals for key disaccharidases.
To analyze the demographic characteristics of patients tested for disaccharidase deficiencies.

Main Methods:

Analysis of 14,827 disaccharidase test results from a laboratory information system.
Application of the Hoffman method to calculate enzyme reference intervals.
Comparison of calculated lower limits with historical diagnostic cutoffs.

Main Results:

The study analyzed data from 14,827 patients, with a median age of 13 years.
New cutoffs were established: lactase 10, maltase 100, palatinase 9, and sucrase 25 U/g protein.
Lactase deficiency was observed in 35% of patients, with 8% having pandisaccharidase deficiency.

Conclusions:

Disaccharidase testing is predominantly performed on pediatric patients (<18 years).
Lactase deficiency is the most prevalent single enzyme deficiency identified.
Historical maltase and sucrase cutoffs are validated; palatinase and lactase cutoffs require adjustment for improved accuracy.