Biodegradable Harmonophores for Targeted High-Resolution In Vivo Tumor Imaging

Area of Science:

• Biomedical Optics
• Nanotechnology
• Cancer Research

Background:

• Nonlinear optical imaging probes, like second harmonic generating (SHG) nanoprobes, are valuable for disease detection due to minimal background signal and high photostability.
• Inorganic SHG nanoprobes often contain transition metals, raising concerns about long-term toxicity.
• Biomedical contrast agents should ideally degrade in vivo without adverse effects.

Purpose of the Study:

• To develop biodegradable optical imaging probes (bioharmonophores) for enhanced cancer detection.
• To create a safer alternative to inorganic nanoprobes with improved in vivo degradability.
• To enable targeted, high-resolution optical imaging for cancer diagnostics and therapy.

Main Methods:

• Development of polymer-encapsulated, self-assembling peptides that generate strong second harmonic generation (SHG) signals.
• Functionalization of bioharmonophores with tumor cell surface markers for targeted delivery.
• In vivo imaging of bioharmonophores in zebrafish embryos to assess targeting and detection sensitivity.

Main Results:

• Successfully developed biodegradable harmonophores (bioharmonophores) with strong SHG signal generation.
• Demonstrated targeted delivery and high-sensitivity detection of single cancer cells in zebrafish embryos.
• Validated the potential of bioharmonophores as safe and effective optical imaging agents.

Conclusions:

• Biodegradable harmonophores represent a promising advancement in optical imaging for cancer diagnostics.
• These peptide-based probes offer a safer, targeted approach for high-resolution in vivo imaging.
• Bioharmonophores pave the way for innovative cancer treatment strategies combining diagnostics and therapy.