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Correlation between lead speciation and inhalation bioaccessibility using two different simulated lung fluids.

Farzana Kastury1, Ranju R Karna2, Kirk G Scheckel3

  • 1Future Industries Institute, University of South Australia, Mawson Lakes Campus, Adelaide, SA, 5095, Australia.

Environmental Pollution (Barking, Essex : 1987)
|February 26, 2021
PubMed
Summary
This summary is machine-generated.

Lead speciation in mining-impacted particulate matter (PM10) affects its bioaccessibility in simulated lung fluids. Different lead (Pb) species showed varying correlations with Pb solubility in vitro, impacting inhalation exposure risk assessment.

Keywords:
ALFDustEXAFSPM10PbSimulated lung fluid

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Area of Science:

  • Environmental Science
  • Toxicology
  • Geochemistry

Background:

  • Particulate matter (PM10) from mining and smelting sites can contain significant lead (Pb) concentrations.
  • Understanding lead speciation is crucial for assessing inhalation exposure risks.
  • In vitro bioaccessibility assays using simulated lung fluids help predict lead's behavior in the respiratory system.

Purpose of the Study:

  • To investigate the relationship between lead speciation in PM10 from Australian mining-impacted soils and its bioaccessibility in simulated lung fluids.
  • To assess the influence of different simulated lung fluids (Hatch's solution and artificial lysosomal fluid) on lead bioaccessibility.
  • To correlate specific lead species with their bioaccessibility percentages.

Main Methods:

  • Extended X-ray Absorption Fine Structure (EXAFS) spectroscopy was used to determine lead speciation in PM10 samples.
  • In vitro bioaccessibility assays were conducted using Hatch's solution (pH 7.4) and artificial lysosomal fluid (ALF, pH 4.5).
  • Scanning Electron Microscopy (SEM) and Energy Dispersive X-ray Spectroscopy (EDX) analyzed elemental composition before and after bioaccessibility assays.

Main Results:

  • Lead bioaccessibility was significantly higher in ALF (72.9-96.3%) compared to Hatch's solution (24.4-48.4%).
  • In Hatch's solution, bioaccessibility positively correlated with anglesite and negatively correlated with lead phosphate and organic-bound lead.
  • No correlation was found between bioaccessibility in ALF and any specific lead species, or between Hatch's solution bioaccessibility and Pb adsorbed onto clay/oxide.

Conclusions:

  • Lead speciation significantly influences its in vitro bioaccessibility, with different simulated lung fluids yielding varied results.
  • The choice of simulated lung fluid is critical for accurate assessment of inhalation exposure risk from lead-containing particulate matter.
  • Further research is needed to fully elucidate the complex interactions between lead speciation and bioaccessibility in respiratory environments.