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Updated: Nov 15, 2025

Microcrystal Electron Diffraction of Small Molecules
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Macromolecular crystallography using microcrystal electron diffraction.

Max T B Clabbers1, Hongyi Xu1

  • 1Department of Materials and Environmental Chemistry, Stockholm University, 106 91 Stockholm, Sweden.

Acta Crystallographica. Section D, Structural Biology
|March 1, 2021
PubMed
Summary
This summary is machine-generated.

Microcrystal electron diffraction (MicroED) is a rapidly advancing technique for determining macromolecular structures. Ongoing developments aim to enhance its accuracy and accessibility for broader use in structural biology.

Keywords:
3D electron diffractionelectron crystallographymacromolecular crystallographymethods developmentmicrocrystal electron diffraction

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Area of Science:

  • Structural Biology
  • Biophysics
  • Crystallography

Background:

  • Microcrystal electron diffraction (MicroED) has emerged as a powerful technique for macromolecular structure determination.
  • Since its inception in 2013, MicroED has undergone rapid evolution and demonstrated significant potential.

Purpose of the Study:

  • To provide an overview of the current status and ongoing developments in MicroED.
  • To highlight the future trajectory and potential impact of MicroED in structural biology.

Main Methods:

  • Utilizes microcrystals for electron diffraction data collection.
  • Employs advanced computational methods for structure determination and refinement.

Main Results:

  • MicroED enables atomic resolution structure determination, including visualization of charges and ligand-binding interactions.
  • The method is effective for solving new protein structures via molecular replacement and determining membrane protein structures.
  • Demonstrated capability in solving structures from microcrystals embedded in lipidic mesophases.

Conclusions:

  • MicroED is a rapidly developing method with the potential to complement existing structural biology techniques.
  • Further optimization is needed to improve the accuracy and accessibility of MicroED experiments.
  • MicroED is anticipated to become a robust tool for macromolecular structure determination in the coming years.