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Quantitative evaluation of SARS-CoV-2 inactivation using a deep ultraviolet light-emitting diode
Takeo Minamikawa1,2,3,4, Takaaki Koma5, Akihiro Suzuki6
1Department of Post-LED Photonics Research, Institute of Post-LED Photonics, Tokushima University, 2-1 Minami-Josanjima, Tokushima, Tokushima, 770-8506, Japan. minamikawa.takeo@tokushima-u.ac.jp.
Scientific Reports
|March 4, 2021
Summary
Deep ultraviolet light-emitting diodes (DUV-LEDs) can inactivate SARS-CoV-2. This study quantifies the DUV-LED irradiation doses needed to eliminate 99.9% of the virus at different wavelengths.
Area of Science:
- Microbiology
- Virology
- Public Health
Background:
- Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes COVID-19, necessitating effective inactivation technologies.
- Deep ultraviolet (DUV) light exhibits known antiviral properties, making DUV-light-emitting diodes (DUV-LEDs) a potential tool for SARS-CoV-2 inactivation.
- Quantitative data on DUV-LED inactivation specific to SARS-CoV-2 is crucial due to variations in viral susceptibility.
Purpose of the Study:
- To quantify the specific DUV-LED irradiation doses required for SARS-CoV-2 inactivation.
- To evaluate the efficacy of DUV-LEDs at different wavelengths (265 nm, 280 nm, 300 nm) against SARS-CoV-2.
- To establish a foundational understanding for DUV-LED based inactivation strategies.
Main Methods:
- Optimized irradiation apparatus and identified suitable culture media for SARS-CoV-2 exposure to DUV-LEDs.
- Exposed SARS-CoV-2 to DUV-LED irradiation at center wavelengths of 265 nm, 280 nm, and 300 nm.
- Quantified the relationship between DUV-LED irradiation dose and SARS-CoV-2 infectivity, controlling for biological variables.
Main Results:
- Determined the precise DUV-LED doses needed to achieve 99.9% inactivation of SARS-CoV-2.
- Identified specific effective doses: 1.8 mJ/cm² at 265 nm, 3.0 mJ/cm² at 280 nm, and 23 mJ/cm² at 300 nm.
- Demonstrated wavelength-dependent inactivation efficacy of DUV-LEDs against SARS-CoV-2.
Conclusions:
- DUV-LED irradiation provides a quantifiable method for SARS-CoV-2 inactivation.
- The study provides essential data for developing and implementing DUV-LED based disinfection technologies against SARS-CoV-2.
- Results contribute to the knowledge base for mitigating COVID-19 transmission through effective inactivation methods.
