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Updated: Nov 15, 2025

Choice and No-Choice Assays for Testing the Resistance of A. thaliana to Chewing Insects
Published on: May 14, 2008
Insect Feeding Assays with Spodoptera exigua on Arabidopsis thaliana
Yanrong You1,2, Chunpeng An1,2, Chuanyou Li1,2
1State Key Laboratory of Plant Genomics, National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Innovation Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, China.
Abstract:
Plant-insect interaction is an important field for studying plant immunity. The beet armyworm, Spodoptera exigua, is one of the best-known agricultural pest insects and is usually used to study plant interactions with chewing insects. Here, we describe a protocol for insect feeding assays with Spodoptera exigua lavae using model host plant Arabidopsis thaliana, which is simple and easy to conduct, and can be used to evaluate the effect of host genes on insect growth and thus to study plant resistance to chewing insects.

