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Updated: Nov 15, 2025

Development of Targeting Induced Local Lesions IN Genomes TILLING Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Development of Targeting Induced Local Lesions IN Genomes TILLING Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Published on: July 16, 2019

The consensus rye microsatellite map with EST-SSRs transferred from wheat.

D O Vidakovic¹, D Perovic², T V Semilet³

¹Julius Kuehn-Institute (JKI), Quedlinburg, Germany University of Novi Sad, Department of Biology and Ecology, Novi Sad, Serbia.

Vavilovskii Zhurnal Genetiki I Selektsii

|March 4, 2021

Summary

This summary is machine-generated.

This study integrated wheat EST-SSRs into rye genetic maps, successfully mapping 23 loci. A consensus rye map was constructed, enhancing genetic resources for rye and wheat breeding.

Keywords:

SSR Secale cereale Triticum aestivum genetic mapping microsatellite markers

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Last Updated: Nov 15, 2025

Development of Targeting Induced Local Lesions IN Genomes TILLING Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

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A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel

A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel

Published on: April 20, 2018

Area of Science:

Plant genetics
Genomics
Molecular biology

Background:

Microsatellite (SSR) markers are crucial for gene mapping in rye, wheat-rye translocations, and triticale.
Existing rye chromosome maps have limited SSR loci, necessitating the integration of new markers.
Wheat EST-SSRs offer a potential source for novel SSR markers in rye.

Purpose of the Study:

To integrate wheat EST-SSRs (Expressed Sequence Tag-derived SSRs) into existing rye genetic maps.
To construct a comprehensive consensus rye microsatellite map.
To expand the available SSR markers for rye chromosome mapping.

Main Methods:

Utilized four rye mapping populations (P87/P105, N6/N2, N7/N2, N7/N6).
Employed CFE (EST-SSRs) primers for mapping.
Integrated mapping data to construct a consensus map.

Main Results:

Successfully mapped 23 Xcfe loci (wheat EST-SSRs) to specific rye chromosomes (1R-7R).
Identified orthologous positions for most mapped Xcfe loci relative to common wheat, considering evolutionary translocations.
Constructed a consensus rye microsatellite map comprising 123 SSRs, 12 SNPs, 118 RFLPs, and 2 isozyme loci.

Conclusions:

The integration of wheat EST-SSRs significantly enriches the rye genetic map.
The developed consensus map provides a valuable resource for gene mapping and QTL analysis in rye.
Orthologous SSR loci facilitate comparative genomics between wheat and rye.