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Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
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Dynamically Tunable Ultrathin Protein Membranes for Controlled Molecular Separation.

Ruyu Li1, Jiayun Xu1,2, Tingting Wang1

  • 1State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, 2699 Qianjin Street, Changchun 130012, China.

ACS Applied Materials & Interfaces
|March 5, 2021
PubMed
Summary

Researchers developed an ultrathin protein membrane using bovine serum albumin (BSA) for efficient molecular separation. This tunable membrane offers high permeance and selectivity, advancing separation technologies.

Keywords:
dynamically tunable membranehigh-performance separationinterfacial self-assemblyprotein self-assemblyselective molecular separation

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Area of Science:

  • Materials Science
  • Biomaterials Engineering
  • Separation Science

Background:

  • Achieving both high permeance and selectivity in ultrathin membranes for separation remains a significant challenge.
  • Existing membrane technologies often face trade-offs between flux and separation efficiency.

Purpose of the Study:

  • To fabricate a large-area, ultrathin protein membrane with tunable separation capabilities.
  • To investigate the performance of protein-based membranes for separating diverse molecules.

Main Methods:

  • Interfacial self-assembly of bovine serum albumin (BSA) and surfactants at an oil/water emulsion interface.
  • Utilizing the unique structure of protein-surrounded channels for molecular sieving.
  • Employing protein folding/unfolding transitions to dynamically regulate pore size.

Main Results:

  • The fabricated ultrathin protein membrane exhibited ultrahigh permeation flux.
  • Demonstrated selective sieving for molecules ranging from dyes to proteins via a dual filtration mechanism.
  • Achieved reversible control over rejection precision by tuning protein conformation under pressure.

Conclusions:

  • The developed dynamically tunable ultrathin protein membrane integrates high permeance, selectivity, and controllability.
  • The membrane shows mechanical stability and recyclability, offering potential for advanced separation applications.
  • This biomimetic approach opens new avenues for high-performance separation technologies.