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[A simple and fast enzyme immunoassay for the quantitative determination of C-reactive protein]
V W Schössler1, G Töpfer, H J Rüger
1Staatliches Institut für Immunpräparate und Nährmedien, Berlin, DDR.
Abstract:
The principle is based on the competitive binding of the enzyme-labelled CRP (enzyme: alkaline phosphatase) and the serum CRP to solid phase-bound antibodies. The detection limit was 10 micrograms/l. The precision in the series in the border-line "normal" to "pathological" (5 mg/l) was 2.9%; the precision from day to day at the concentration of 5 mg/l was 4.5%. Only 2 h are required for the whole test.