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Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Confocal Fluorescence Microscopy01:16

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

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Related Experiment Video

Updated: Jun 27, 2026

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IoT-Based Research Equipment Sharing System for Remotely Controlled Two-Photon Laser Scanning Microscopy.

Eunwoo Park1,2, Jaehyun Lim2, Byung Cheol Park3

  • 1Advanced Photonics Research Institute, Gwangju Institute of Science and Technology, Gwangju 61005, Korea.

Sensors (Basel, Switzerland)
|March 6, 2021
PubMed
Summary

This study introduces an Internet of Things (IoT)-based two-photon laser scanning microscopy (TPLSM) system for remote equipment sharing. The IoT-TPLSM system demonstrates comparable performance to conventional TPLSM, enabling remote research access.

Keywords:
IoTMQTTremote controlremote operationremote sharing economyresearch equipment sharingtwo-photon laser scanning microscopy

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Area of Science:

  • Microscopy
  • Internet of Things (IoT)
  • Biotechnology

Background:

  • Traditional two-photon laser scanning microscopy (TPLSM) systems are often limited by physical accessibility, hindering collaborative research and resource sharing.
  • The integration of the Internet of Things (IoT) offers a potential solution for overcoming these limitations by enabling remote access and control of scientific equipment.

Purpose of the Study:

  • To develop and evaluate an IoT-based TPLSM system (IoT-TPLSM) for remote research equipment sharing.
  • To demonstrate the feasibility and performance of the IoT-TPLSM for acquiring high-quality biological images remotely.

Main Methods:

  • The conventional data acquisition (DAQ) system in TPLSM was replaced with IoT modules for remote data transmission and reception via a web service.
  • A Message Queuing Telemetry Transport (MQTT) protocol was implemented for the DAQ interface, and a graphical user interface (GUI) was developed for remote operation.
  • Performance of individual IoT modules was assessed, including operation in a personal computer-free environment.

Main Results:

  • The IoT-TPLSM system successfully acquired stained cellular and autofluorescent tissue images remotely.
  • The system demonstrated comparable performance to conventional TPLSM in terms of imaging conditions and the quality of processed 3D image stacks.
  • Individual IoT modules performed well, functioning effectively even without a connected personal computer.

Conclusions:

  • The proposed IoT-TPLSM system effectively enables remote research equipment sharing, facilitating a remote sharing economy for advanced microscopy.
  • The system provides a viable platform for remote operation and image acquisition with performance comparable to traditional TPLSM.
  • This technology has the potential to increase accessibility and utilization of sophisticated microscopy tools in scientific research.