Current trends and challenges in analysis and characterization of engineered nanoparticles in seawater

With the increasingly wide use of engineered nanoparticles (ENPs), their release into the environment makes it important to determine in what quantitates they occur in aquatic systems and to understand their fate therein. In particular, detection and quantification of ENPs in seawater is challenging and often requires analytical methods to perform close to the feasibility confines. This review is aimed at critical analysis of current and emerging capabilities of analytical methods as have been employed for the analysis and characterization of ENPs in seawater in the last decade. An emphasis is given to the most reliable experimental strategies focused on avoiding the high-salt matrix effect and isolation and enrichment of the nanoparticulate fraction prior to analysis. Advanced analytical methodology in use basically relies on the application of elemental mass spectrometry to determine various particle-core metals and its single-particle mode to characterize the seawater-mediated transformation of ENPs, including dissolution, aggregation, etc. On the other hand, common microscopy, light scattering or X-ray based techniques are not sensitive enough to acquire the transformation information from real seawater samples. Finally, attention is pinpointed upon an acute shortcoming of the current research which is in the overwhelming majority of cases restricted to samples spiked with ENPs and often at excessive concentration levels.